Reconstitution of acetosyringone-mediated Agrobacterium tumefaciens virulence gene expression in the heterologous host Escherichia coli

Research demonstrates that virA and virG virulence genes of Agrobacterium tumefaciens can be successfully reconstituted in Escherichia coli in a inducer-dependent lac promoter-driven approach. Results show that the C-terminal domain of RpoA is necessary for interaction with the transcriptional activator VirG in the above fusion strategy.