Effect of temperature, pH, and metal ion binding on the secondary structure of bacteriorhodopsin: FT-IR study of the melting and premelting transition temperatures

Research has been conducted on the bacteriorhodopsin. The temperature dependence of the bacteriorhodopsin's FT-IR spectra as a function of pH has been examined and the results indicate a strong dependence of the irreversible melting transition on the pH of the native bacteriorhodopsin.