Preparation of (R)- and (S)-N-protected 3-hydroxypyrrolidines by hydroxylation with Sphingomonas sp. HXN-200, a highly active, regio- and stereoselective, and easy to handle biocatalyst

Research has been conducted on the N-benzylpyrrolidine. The hydroxylation of this compound has been carried out with the Sphingomonas sp. HXN-200 resting cells to produce N-benzyl-3-hydroxypyrrolidine and the results indicate that the change of the docking/protecting group in pyrrolidines improves the hydroxylation activity and enantioselectivity of the compound.