Analysis of NSF mutants reveals residues involved in SNAP binding and ATPase stimulation

Authors :: Horsnell, William G.C.
Steel, Gregor J.
Morgan, Alan
Source :: Biochemistry. April 23, 2002, Vol. 41 Issue 16, p5230, 6 p.
Publication Year :: 2002
Abstract: In Drosophila comatose, the sec 18-1 mutation, in terms of G89D substitution in the Sec 18p domain, affects the protein's ability to bind to Sec17p of the yeast soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein. Data suggest that NSF protein binds to soluble NSF attachment protein and it is dependent on ATPase stimulation.

Subjects :: Membrane fusion -- Physiological aspects
Mutation (Biology) -- Analysis
Protein binding -- Analysis
Enzymes -- Structure-activity relationship
Biological sciences
Chemistry

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