Analysis of the DNA-binding properties of MyoD, myogenin, and E12 by fluorescence anisotropy

Authors :: Maleki, Soheila J.
Royer, Catherine A.
Hurlburt, Barry K.
Source :: Biochemistry. Sept 3, 2002, Vol. 41 Issue 35, p10888, 7 p.
Publication Year :: 2002
Abstract: Results reveal that the DNA serves as an allosteric ligand promoting the dimerization of MyoD, myogenin, and E12 as shown by fluorescence anisotropy assays. The heterodimers mediate transcriptional regulation. Research demonstrates that preferential heterodimerization of the myogenic regulatory factors possessing an E-protein drive muscle-specific gene expression during myogenesis.

Subjects :: DNA binding proteins -- Physiological aspects
Genetic transcription -- Regulation
Myogenesis -- Physiological aspects
Proteins -- Structure
Biological sciences
Chemistry

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