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Iron regulates xanthine oxidase activity in the lung
- Source :
- The American Journal of Physiology. Sept, 2002, Vol. 283 Issue 3, pL563, 10 p.
- Publication Year :
- 2002
-
Abstract
- The iron chelator deferoxamine has been reported to inhibit both xanthine oxidase (XO) and xanthine dehydrogenase activity, but the relationship of this effect to the availability of iron in the cellular and tissue environment remains unexplored. XO and total xanthine oxidoreductase activity in cultured V79 cells was increased with exposure to ferric ammonium sulfate and inhibited by deferoxamine. Lung XO and total xanthine oxidoreductase activities were reduced in rats fed an iron-depleted diet and increased in rats supplemented with iron, without change in the ratio of XO to total oxidoreductase. Intratracheal injection of an iron salt or silica-iron, but not aluminum salts or silica-zinc, significantly increased rat lung XO and total xanthine oxidoreductase activities, immunoreactive xanthine oxidoreductase, and the concentration of urate in bronchoalveolar fluid. These results suggest the possibility that the production of uric acid, a major chelator of iron in extracellular fluid, is directly influenced by iron-mediated regulation of the expression and/or activity of its enzymatic source, xanthine oxidase. xanthine dehydrogenase; deferoxamine; silica; uric acid
- Subjects :
- Molecular biology -- Research
Iron in the body -- Physiological aspects
Deferoxamine -- Physiological aspects
Oxidases -- Physiological aspects
Uric acid -- Physiological aspects
Chelates -- Physiological aspects
Silica -- Physiological aspects
Enzymes -- Physiological aspects
Lungs -- Physiological aspects
Rats -- Physiological aspects
Xanthine -- Physiological aspects
Biological sciences
Subjects
Details
- ISSN :
- 00029513
- Volume :
- 283
- Issue :
- 3
- Database :
- Gale General OneFile
- Journal :
- The American Journal of Physiology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.92685639