Accumulation of 2-aminophenoxazin-3-one-7-carboxylate during growth of Pseudomonas putida TW3 on 4-nitro-substituted substrates requires 4-hydroxylaminobenzoate lyase (PnbB)

Results show that 2-aminophenoxazin-3-one-7-carboxylate is formed via oxidative dimerization of 4-amino-3-hydroxybenzoate, which undergoes replacement of the 4-amino group by a hydroxyl to form protocatechuate. Data indicate that 4-hydroxylaminobenzoate lyase converts 4-hydroxylaminobenzoate to 3,4-dihydroxybenzoate (protocatechuate).