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Dynactin is required for bidirectional organelle transport
- Source :
- The Journal of Cell Biology. Feb 3, 2003, Vol. 160 Issue 3, p297, 5 p.
- Publication Year :
- 2003
-
Abstract
- Kinesin II is a heterotrimeric plus end-directed microtubule motor responsible for the anterograde movement of organelles in various cell types. Despite substantial literature concerning the types of organelles that kinesin II transports, the question of how this motor associates with cargo organelles remains unanswered. To address this question, we have used Xenopus laevis melanophores as a model system. Through analysis of kinesin II-mediated melanosome motility, we have determined that the dynactin complex, known as an anchor for cytoplasmic dynein, also links kinesin II to organelles. Biochemical data demonstrates that the putative cargo-binding subunit of Xenopus kinesin II, Xenopus kinesin II-associated protein (XKAP), binds directly to the p[150.sup.Glued] subunit of dynactin. This interaction occurs through aa 530-793 of XKAP and aa 600-811 of p[150.sup.Glued]. These results reveal that dynactin is required for transport activity of microtubule motors of opposite polarity, cytoplasmic dynein and kinesin II, and may provide a new mechanism to coordinate their activities.
- Subjects :
- Cell research -- Analysis
Kinesin -- Genetic aspects
Kinesin -- Physiological aspects
Microtubules -- Genetic aspects
Microtubules -- Physiological aspects
Cell organelles -- Genetic aspects
Cell organelles -- Physiological aspects
Cytoplasm -- Genetic aspects
Cytoplasm -- Physiological aspects
Biochemistry -- Research
Proteins -- Genetic aspects
Proteins -- Physiological aspects
Biological sciences
Subjects
Details
- ISSN :
- 00219525
- Volume :
- 160
- Issue :
- 3
- Database :
- Gale General OneFile
- Journal :
- The Journal of Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.98882721