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Large-Scale Hematopoietic Differentiation of Human Induced Pluripotent Stem Cells Provides Granulocytes or Macrophages for Cell Replacement Therapies
- Source :
- Lachmann, N., M. Ackermann, E. Frenzel, S. Liebhaber, S. Brennig, C. Happle, D. Hoffmann, et al. 2015. “Large-Scale Hematopoietic Differentiation of Human Induced Pluripotent Stem Cells Provides Granulocytes or Macrophages for Cell Replacement Therapies.” Stem Cell Reports 4 (2): 282-296. doi:10.1016/j.stemcr.2015.01.005. http://dx.doi.org/10.1016/j.stemcr.2015.01.005.
- Publication Year :
- 2015
- Publisher :
- Elsevier, 2015.
-
Abstract
- Summary Interleukin-3 (IL-3) is capable of supporting the proliferation of a broad range of hematopoietic cell types, whereas granulocyte colony-stimulating factor (G-CSF) and macrophage CSF (M-CSF) represent critical cytokines in myeloid differentiation. When this was investigated in a pluripotent-stem-cell-based hematopoietic differentiation model, IL-3/G-CSF or IL-3/M-CSF exposure resulted in the continuous generation of myeloid cells from an intermediate myeloid-cell-forming complex containing CD34+ clonogenic progenitor cells for more than 2 months. Whereas IL-3/G-CSF directed differentiation toward CD45+CD11b+CD15+CD16+CD66b+ granulocytic cells of various differentiation stages up to a segmented morphology displaying the capacity of cytokine-directed migration, respiratory burst response, and neutrophil-extracellular-trap formation, exposure to IL-3/M-CSF resulted in CD45+CD11b+CD14+CD163+CD68+ monocyte/macrophage-type cells capable of phagocytosis and cytokine secretion. Hence, we show here that myeloid specification of human pluripotent stem cells by IL-3/G-CSF or IL-3/M-CSF allows for prolonged and large-scale production of myeloid cells, and thus is suited for cell-fate and disease-modeling studies as well as gene- and cell-therapy applications.
Details
- Language :
- English
- ISSN :
- 22136711
- Database :
- Digital Access to Scholarship at Harvard (DASH)
- Journal :
- Lachmann, N., M. Ackermann, E. Frenzel, S. Liebhaber, S. Brennig, C. Happle, D. Hoffmann, et al. 2015. “Large-Scale Hematopoietic Differentiation of Human Induced Pluripotent Stem Cells Provides Granulocytes or Macrophages for Cell Replacement Therapies.” Stem Cell Reports 4 (2): 282-296. doi:10.1016/j.stemcr.2015.01.005. http://dx.doi.org/10.1016/j.stemcr.2015.01.005.
- Publication Type :
- Academic Journal
- Accession number :
- edshld.1.14065550
- Document Type :
- Journal Article
- Full Text :
- https://doi.org/10.1016/j.stemcr.2015.01.005