Limits to Resolution of CW STED Microscopy

Authors :: Trifonov, Alexei
Jaskula, Jean-Christophe
Teulon, Claire
Glenn, David R.
Bar-Gill, Nir
Walsworth, Ronald Lee
Source :: Trifonov, Alexei S., Jean-Christophe Jaskula, Claire Teulon, David R. Glenn, Nir Bar-Gill, and Ronald L. Walsworth. 2013. Limits to Resolution of CW STED Microscopy. In Volume 62 of Advances in Atomic, Molecular, and Optical Physics, ed. Paul Berman, Ennio Arimondo, and Chun Lin, 279–302. Chennai: Academic Press.
Publication Year :: 2013
Publisher :: Academic Press (Elsevier BV), 2013.
Abstract: We report a systematic theoretical and experimental study of the limits to spatial resolution for stimulated emission depletion (STED) superresolution fluorescence microscopy using continuous wave (CW) laser beams. We develop a theoretical framework for CW STED imaging from point fluorescent emitters and calculate the dependence of 2D spatial resolution on the power of the CW excitation (pump) beam, as well as the power, contrast, and polarization of the CW STED “doughnut” beam. We perform CW STED experiments on (non-bleaching) nitrogen vacancy (NV) color centers in diamond and find good agreement with the theoretical expressions for CW STED spatial resolution. Our results will aid the optimization and application of CW STED microscopy in both the physical and life sciences.<br />Physics

Language :: English
ISBN :: 978-0-12-408090-4
0-12-408090-1
ISSN :: 1049250X
ISBNs :: 9780124080904 and 0124080901
Database :: Digital Access to Scholarship at Harvard (DASH)
Journal :: Trifonov, Alexei S., Jean-Christophe Jaskula, Claire Teulon, David R. Glenn, Nir Bar-Gill, and Ronald L. Walsworth. 2013. Limits to Resolution of CW STED Microscopy. In Volume 62 of Advances in Atomic, Molecular, and Optical Physics, ed. Paul Berman, Ennio Arimondo, and Chun Lin, 279–302. Chennai: Academic Press.
Publication Type :: Book
Accession number :: edshld.1.34268563
Document Type :: Monograph or Book
Full Text :: https://doi.org/10.1016/B978-0-12-408090-4.00005-0