Effects of green tea catechins and broccoli extracts on the antioxidant enzymes and life span of Drosophila melanogaster.

Li, Yuk Man.
Thesis (M.Phil.)--Chinese University of Hong Kong, 2005.
Includes bibliographical references (leaves 121-132).
s in English and Chinese.
ACKNOWLEDGMENTS --- p.i
p.ii
LIST OF ABBREVATIONS --- p.vii
TABLE OF CONTENTS --- p.x
Chapter Chapter 1 --- General Introduction
Chapter 1.1 --- Introduction --- p.1
Chapter 1.2 --- Theories of aging --- p.2
Chapter 1.2.1 --- Free radical theory of aging --- p.3
Chapter 1.2.2 --- Free radicals reactions and their effects --- p.4
Chapter 1.2.2.1 --- Lipid peroxidation --- p.6
Chapter 1.2.2.2 --- Protein oxidation --- p.6
Chapter 1.2.2.3 --- DNA oxidation --- p.8
Chapter 1.2.2.4 --- Carbohydrate oxidation --- p.8
Chapter 1.2.3 --- Antioxidant defense systems --- p.9
Chapter 1.2.3.1 --- Superoxide dismutase (SOD) --- p.9
Chapter 1.2.3.2 --- Catalase --- p.11
Chapter 1.2.3.3 --- Glutathione peroxidase (GPx) and reductase --- p.11
Chapter 1.3 --- Models on aging study --- p.12
Chapter 1.3.1 --- Non-mammalian model systems --- p.12
Chapter 1.3.1.1 --- History of Drosophila on longevity study --- p.14
Chapter 1.3.1.2 --- Advantages of Drosophila model --- p.14
Chapter 1.3.2 --- Mammalian model systems --- p.15
Chapter 1.4 --- Antioxidant effects on aging process --- p.16
Chapter 1.4.1 --- Antioxidant capacity of tea --- p.16
Chapter 1.4.2 --- Antioxidant capacity of vegetables --- p.19
Chapter 1.5 --- Dietary fat on longevity --- p.21
Chapter Chapter 2 --- Anti-aging: Effects of Green Tea Catechins on Life Span and Antioxidant Enzymes of Drosophila melanogaster
Chapter 2.1 --- Introduction --- p.24
Chapter 2.2 --- Gbjectives --- p.27
Chapter 2.3 --- Materials and methods --- p.28
Chapter 2.3.1 --- Materials --- p.28
Chapter 2.3.2 --- Preparation of GTCs --- p.28
Chapter 2.3.3 --- Feeding experiment --- p.30
Chapter 2.3.3.1 --- Dose determination --- p.31
Chapter 2.3.3.2 --- Paraquat treatment --- p.31
Chapter 2.3.3.3 --- Hydrogen peroxide (H202) treatment --- p.33
Chapter 2.3.3.4 --- Enzyme activity assay --- p.34
Chapter 2.3.4 --- Enzyme analysis --- p.34
Chapter 2.3.4.1 --- SOD activity --- p.34
Chapter 2.3.4.2 --- Catalase activity --- p.35
Chapter 2.3.4.3 --- Lipid hydorperoxies (LOOHs) formation --- p.36
Chapter 2.3.4.4 --- Determination of protein concentration --- p.37
Chapter 2.3.5 --- Semi-quantitative RT-PCR assay --- p.37
Chapter 2.3.5.1 --- RNA isolation --- p.37
Chapter 2.3.5.2 --- Primer sequences --- p.38
Chapter 2.3.5.3 --- Reverse Transcriptase PCR --- p.40
Chapter 2.3.6 --- Statistics --- p.40
Chapter 2.4 --- Results --- p.42
Chapter 2.4.1 --- Dose determination --- p.42
Chapter 2.4.2 --- Paraquat treatment --- p.45
Chapter 2.4.3 --- H202 treatment --- p.48
Chapter 2.4.4 --- SOD activity --- p.51
Chapter 2.4.5 --- Catalase activity --- p.51
Chapter 2.4.6 --- LOOHs formation --- p.53
Chapter 2.4.7 --- Semi-quantitative RT-PCR assay --- p.53
Chapter 2.5 --- Discussion --- p.55
Chapter Chapter 3 --- Anti-aging: Effects of Vegetable Extracts on Life Span and Antioxidant Enzymes of Drosophila melanogaster
Chapter 3.1 --- Introduction --- p.62
Chapter 3.2 --- Objectives --- p.64
Chapter 3.3 --- Materials and methods --- p.65
Chapter 3.3.1 --- Materials --- p.65
Chapter 3.3.2 --- Preparation of vegetables extracts --- p.65
Chapter 3.3.3 --- Feeding experiment --- p.65
Chapter 3.3.3.1 --- Dose determination --- p.66
Chapter 3.3.3.2 --- Paraquat and hydrogen peroxide (H2O2) treatment --- p.67
Chapter 3.3.4 --- Lipid hydroperoxide (LOOHs) formation --- p.67
Chapter 3.3.5 --- Enzyme analysis --- p.67
Chapter 3.3.6 --- Semi-quantitative RT-PCR assay --- p.67
Chapter 3.3.7 --- Statistics --- p.68
Chapter 3.4 --- Results --- p.69
Chapter 3.4.1 --- Dose determination --- p.69
Chapter 3.4.2 --- Antioxidant effect among different vegetables --- p.69
Chapter 3.4.3 --- SOD activity --- p.76
Chapter 3.4.4 --- Catalase activity --- p.76
Chapter 3.4.5 --- LOOHs formation --- p.78
Chapter 3.4.6 --- Semi-quantitative RT-PCR assay --- p.78
Chapter 3.5 --- Discussion --- p.80
Chapter Chapter 4 --- Anti-aging Activity of Greeen Tea Catechins and Broccoli Extracts in Drosophila melanogaster Fed a High Fat Diet
Chapter 4.1 --- Introduction --- p.85
Chapter 4.2 --- Objectives --- p.87
Chapter 4.3 --- Materials and methods --- p.88
Chapter 4.3.1 --- Materials --- p.88
Chapter 4.3.2 --- Preparation of free fatty acid --- p.88
Chapter 4.3.3 --- Prepartion of GTCs and BEs --- p.89
Chapter 4.3.4 --- Feeding experiment --- p.89
Chapter 4.3.5 --- LOOHs formation --- p.90
Chapter 4.3.6 --- Enzyme analysis --- p.91
Chapter 4.3.7 --- Semi-quantitative RT-PCR assay --- p.91
Chapter 4.3.8 --- Statistics --- p.91
Chapter 4.4 --- Results --- p.92
Chapter 4.4.1 --- Dose determination --- p.92
Chapter 4.4.2 --- Effects of GTCs or BEs on life span with lard consumption --- p.95
Chapter 4.4.3 --- LOOHs formation --- p.101
Chapter 4.4.4 --- SOD activity --- p.103
Chapter 4.4.5 --- Catalase activity --- p.107
Chapter 4.4.6 --- Semi-quantitative RT-PCR assay --- p.110
Chapter 4.5 --- Discussion --- p.112
References --- p.121
http://library.cuhk.edu.hk/record=b5892503
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