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Imaging Renal Urea Handling in Rats at Millimeter Resolution using Hyperpolarized Magnetic Resonance Relaxometry.

Imaging Renal Urea Handling in Rats at Millimeter Resolution using Hyperpolarized Magnetic Resonance Relaxometry.

Authors :
Reed, GD
von Morze, C
Verkman, AS
Koelsch, BL
Chaumeil, MM
Lustig, M
Ronen, SM
Bok, RA
Sands, JM
Larson, PEZ
Wang, ZJ
Larsen, JHA
Kurhanewicz, J
Vigneron, DB
Reed, GD
von Morze, C
Verkman, AS
Koelsch, BL
Chaumeil, MM
Lustig, M
Ronen, SM
Bok, RA
Sands, JM
Larson, PEZ
Wang, ZJ
Larsen, JHA
Kurhanewicz, J
Vigneron, DB
Source :
Tomography : a journal for imaging research; vol 2, no 2, 125-135; 2379-1381
Publication Year :
2016

Abstract

In vivo spin spin relaxation time (T2) heterogeneity of hyperpolarized [13C,15N2]urea in the rat kidney was investigated. Selective quenching of the vascular hyperpolarized 13C signal with a macromolecular relaxation agent revealed that a long-T2 component of the [13C,15N2]urea signal originated from the renal extravascular space, thus allowing the vascular and renal filtrate contrast agent pools of the [13C,15N2]urea to be distinguished via multi-exponential analysis. The T2 response to induced diuresis and antidiuresis was performed with two imaging agents: hyperpolarized [13C,15N2]urea and a control agent hyperpolarized bis-1,1-(hydroxymethyl)-1-13C-cyclopropane-2H8. Large T2 increases in the inner-medullar and papilla were observed with the former agent and not the latter during antidiuresis. Therefore, [13C,15N2]urea relaxometry is sensitive to two steps of the renal urea handling process: glomerular filtration and the inner-medullary urea transporter (UT)-A1 and UT-A3 mediated urea concentrating process. Simple motion correction and subspace denoising algorithms are presented to aid in the multi exponential data analysis. Furthermore, a T2-edited, ultra long echo time sequence was developed for sub-2 mm3 resolution 3D encoding of urea by exploiting relaxation differences in the vascular and filtrate pools.

Details

Database :
OAIster
Journal :
Tomography : a journal for imaging research; vol 2, no 2, 125-135; 2379-1381
Notes :
125 - 135, application/pdf, Tomography : a journal for imaging research vol 2, no 2, 125-135, 2379-1381, english
Publication Type :
Electronic Resource
Accession number :
edsoai.on1022185354
Document Type :
Electronic Resource