亜急性小脳変性症における血中抗神経組織抗体の検討

This is a report of two patients with subacute cerebellar degeneration associated with cancer whose sera showed antibody activities against nervous tissue elements. Using an immuno-blotting method, the serum of a 68-year-old man with subacute cerebellar degeneration and malignant lymphoma reacted with rat cerebral 250-kd and 110-kd and cerebellar 110-kd acidic cytoplasmic proteins. The serum IgG of the second patient, a 50-year-old woman, who had subacute cerebellar degeneration, Lambert-Eaton myasthenic syndrome and small cell cancer of the lung showed antibody activities to rat cerebral and cerebellar 98-kd neutral cytoplasmic protein and 68-kd membrane protein. These sera did not react with the proteins prepared from human and rat brain obtained several hours after death, which indicated that these antigen proteins were easily degraded and needed to be prepared quickly after death with protease inhibitors. These patients' sera did not react with proteins prepared from rat liver, kidney, spleen, heart muscle and diaphragm and the antigen proteins reacted with these sera might be brain specific. Control sera did not react with any of the brain proteins. The serum of each patients recognized different antigens. Other reported antigens in the literature are also different which indicates those proteins reacting with serum antibody are variable indiviaually among patients with subacute cerebellar degeneration.