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Sensitive detection of mitochondrial DNA variants for analysis of mitochondrial DNA-enriched extracts from frozen tumor tissue
- Publication Year :
- 2018
-
Abstract
- Large variation exists in mitochondrial DNA (mtDNA) not only between but also within individuals. Also in human cancer, tumor-specific mtDNA variation exists. In this work, we describe the comparison of four methods to extract mtDNA as pure as possible from frozen tumor tissue. Also, three state-of-the-art methods for sensitive detection of mtDNA variants were evaluated. The main aim was to develop a procedure to detect low-frequent single-nucleotide mtDNA-specific variants in frozen tumor tissue. We show that of the methods evalua
Details
- Database :
- OAIster
- Notes :
- application/pdf, Scientific Reports vol. 8 no. 1, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1042808452
- Document Type :
- Electronic Resource
- Full Text :
- https://doi.org/10.1038.s41598-018-20623-7