Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system

Krishnendu Manna,1,* Snehasis Mishra,1,* Moumita Saha,1 Supratim Mahapatra,1 Chirag Saha,1 Govind Yenge,2 Nilesh Gaikwad,2 Ramkrishna Pal,2 Dasharath Oulkar,3 Kaushik Banerjee,3 Krishna Das Saha1 1Cancer Biology and Inflammatory Disorder Division, Council of Scientific & Industrial Research–Indian Institute of Chemical Biology, Kolkata 700032, West Bengal, India; 2Postharvest Technology Laboratory, Indian Council of Agricultural Research-National Research Centre on Pomegranate, Solapur 413255, Maharashtra, India; 3National Referral Laboratory, Indian Council of Agricultural Research-National Research Centre for Grapes, Pune 412307, Maharashtra, India *These authors contributed equally to this work Background: Diabetic nephropathy (DN), an end-stage renal disorder, has posed a menace to humankind globally, because of its complex nature and poorly understandable intricate mechanism. In recent times, functional foods as potential health benefits have been gaining attention of consumers and researchers alike. Rich in antioxidants, the peel and seed of pomegranate have previously demonstrated protection against oxidative-stress-related diseases, including cardiovascular disorders, diabetes, and cancer.Purpose: This study was designed to investigate the ameliorative role of pomegranate peel extract–stabilized gold nanoparticle (PPE-AuNP) on streptozotocin (STZ)-induced DN in an experimental murine model.Methods: Following the reduction methods, AuNP was prepared using the pomegranate peel ellagitannins and characterized by particle size, physical appearance, and morphological architecture. Modulatory potential of PPE-AuNP was examined through the plethora of biochemical and high throughput techniques, flow cytometry, immunoblotting, and immunofluorescence.Results: The animals treated with PPE-AuNP markedly reduced the fasting blood glucose, renal toxicity indices, and serum TC and TG in a hyperglycemic condition. As evident from an in