Distribution and characterization of phytopathogenic bacteria on commercial bean crop in Vojvodina

The present paper discusses the occurrence and severity of bacterial bean diseases in the Serbian province of Vojvodina. Leaf samples of diseased bean plants were collected from a number of locations in the province. All the samples from all the locations tested positive for Xanthomonas axonopodis pv. phaseoli, indicating that the bacterium is widespread on commercial plots and gardens planted to bean in Vojvodina. The bacterium Pseudomonas savastanoi pv. phaseolicola was isolated from a smaller number of samples, which shows that it is not widespread on bean in the province. Identification of the isolates was confirmed using the classic phytobacterial methods (for the pathogenic, morphological, breeding and biochemical-physiological characteristics of the isolates) as well as the rapid modern methods (ELISA for serological and PCR for molecular properties).
U radu su proučavane bakterioze pasulja, njihovo prisustvo i intenzitet pojave na teritoriji Vojvodine. Izolacijama iz prikupljenih obolelih uzoraka pasulja (list) na standardne hranljive podloge, dobijene su kolonije Xanthomonas axonopodis pv. phaseoli iz svih ispitivanih uzoraka i lokaliteta, što ukazuje na dominantno prisustvo ove bakterije na proizvodnim parcelama i okućnicama pasulja kod nas. Bakterija Pseudomonas savastanoi pv. phaseolicola je izolovana kod manjeg broja uzoraka, što pokazuje da nije široko rasprostranjena u našim uslovima gajenja pasulja. Identifikacija izolata ovih bakterija je dokazana korišćenjem klasičnih fitobakterioloških (patogene, morfološke, odgajivačke i biohemijsko-fiziološke odlike izolata) i brzih, savremenih metoda (serološke-ELISA i molekularne-PCR).