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Construction and initial characterization of yeast strains with Dyskeratosis congenita mutations

Authors :
Wieden-Kothe, Ute
Ogailan, Abeer Abdullah
University of Lethbridge. Faculty of Arts and Science
Wieden-Kothe, Ute
Ogailan, Abeer Abdullah
University of Lethbridge. Faculty of Arts and Science
Publication Year :
2017

Abstract

The effect that Dyskeratosis congenita mutations have on ribosome biogenesis has not been fully investigated. Here, I am studying the effect of Dyskeratosis congenita mutations using a yeast model system with the goal of identifying impacts on ribosome biogenesis. I constructed six haploid yeast strains with a single mutation in the CBF5 gene each using site-directed mutagenesis and chromosomal integration. First, I screened for phenotypic effects on growth on solid and in liquid medium at different temperatures; these experiments show normal growth behavior of the mutant strains compared to wild type. Second, Cbf5p expression levels were examined in whole-cell extract via western blotting analysis, which again did not reveal any difference to wild type. Finally, progression through the cell cycle was investigated in synchronized cells. Together, this study indicates that individual Dyskeratosis congenita mutations are well tolerated in the unicellular eukaryote Saccharomyces cerevisiae.

Details

Database :
OAIster
Notes :
en_US
Publication Type :
Electronic Resource
Accession number :
edsoai.on1119734547
Document Type :
Electronic Resource