Phytic acid degradation by phytase – as viewed by 31P NMR and multivariate curve resolution

The 31P NMR method is a most direct and useful method to describe the degradation of phytic acid to lower inositol phosphates by the action of the enzyme phytase. The use of chemometric and CARS visualizes and helps in the interpretation of the results. By means of LatentiX it has been possible to visualize the time-dependent hydrolysis of phytic acid and by PCA the complexity of the phytic acid is shown in the score plots. By modeling the spectra in CARS it is possible to identify and quantify each of the inositol phosphates.