Optimization and analysis of lipid nanoparticles for in vivo mRNA delivery

Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2017.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 153-167).
Messenger RNA (mRNA) therapeutics have the potential to treat a diverse array of diseases requiring protein expression, with applications in protein replacement therapies, immunotherapies, and genome engineering. However, the intracellular delivery of mRNA is challenging and necessitates a safe and effective delivery vector. Lipid nanoparticles (LNPs) have shown considerable promise for the delivery of small interfering RNAs (siRNA) to the liver but their utility as agents for mRNA delivery have only been recently investigated. New delivery materials for mRNA delivery are also being developed which have the potential to transfect nonliver targets, but the screening of these vectors in vivo is low-throughput and it is difficult to determine transfected cell types. There is a need both for efficacious, well-characterized mRNA delivery materials and for methods to facilitate in vivo screening of novel materials. We first developed a generalized strategy to optimize LNP formulations for mRNA delivery to the liver using Design of Experiment methodologies. By simultaneously varying lipid ratios and structures, we developed an optimized formulation which increased the potency of eryrthopoietin-mRNA-loaded LNPs in vivo 7-fold relative to formulations previously used for siRNA delivery. Next, we explored the immune response and activity of base-modified LNPformulated mRNA administered systemically in vivo. We observed indications of a previously uncharacterized transient, extracellular innate immune response to mRNA-LNPs, including neutrophilia, myeloid cell activation, and up-regulation of four serum cytokines. Although we have developed a more efficacious liver-targeting LNP, many mRNA therapies will require delivery to non-liver tissues. Using trial-and-error approaches, we discover novel formulations capable of inducing mRNA expression in vivo in the spleen, lung, and fat. To increase the throughput of in vivo screening, we report a new barcoding-based approach capable o
by Kevin John Kauffman.
Ph. D.