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A Method for in Vivo Quantification of Cytokine IL-1β in the Rat Intrathecal Space

Authors :
Deng, F
Arman, A
Goldys, EM
Hutchinson, MR
Liu, G
Deng, F
Arman, A
Goldys, EM
Hutchinson, MR
Liu, G
Publication Year :
2020

Abstract

IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL-1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague-Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.

Details

Database :
OAIster
Publication Type :
Electronic Resource
Accession number :
edsoai.on1157336923
Document Type :
Electronic Resource