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Zinc Finger RNA-Binding Protein Zn72D Regulates ADAR-Mediated RNA Editing in Neurons.

Authors :
Sapiro, Anne L
Freund, Emily C
Restrepo, Lucas
Qiao, Huan-Huan
Bhate, Amruta
Li, Qin
Ni, Jian-Quan
Mosca, Timothy J
Li, Jin Billy
Sapiro, Anne L
Freund, Emily C
Restrepo, Lucas
Qiao, Huan-Huan
Bhate, Amruta
Li, Qin
Ni, Jian-Quan
Mosca, Timothy J
Li, Jin Billy
Source :
Department of Neurology Faculty Papers
Publication Year :
2020

Abstract

Adenosine-to-inosine RNA editing, catalyzed by adenosine deaminase acting on RNA (ADAR) enzymes, alters RNA sequences from those encoded by DNA. These editing events are dynamically regulated, but few trans regulators of ADARs are known in vivo. Here, we screen RNA-binding proteins for roles in editing regulation with knockdown experiments in the Drosophila brain. We identify zinc-finger protein at 72D (Zn72D) as a regulator of editing levels at a majority of editing sites in the brain. Zn72D both regulates ADAR protein levels and interacts with ADAR in an RNA-dependent fashion, and similar to ADAR, Zn72D is necessary to maintain proper neuromuscular junction architecture and fly mobility. Furthermore, Zn72D's regulatory role in RNA editing is conserved because the mammalian homolog of Zn72D, Zfr, regulates editing in mouse primary neurons. The broad and conserved regulation of ADAR editing by Zn72D in neurons sustains critically important editing events.

Details

Database :
OAIster
Journal :
Department of Neurology Faculty Papers
Notes :
application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1159990590
Document Type :
Electronic Resource