腫節風藥材的HPLC指紋圖譜研究及其異嗪皮啶與迷迭香酸的含量測定

目的:研究腫節風藥材的高效液相色譜(HPLC)指紋圖譜,并測定藥材中異嗪皮啶與迷迭香酸的含量,以建立藥材有效的質量評價方法。方法:色譜柱為Inertsil ODS-4(250 mm×4.6 mm,5μm),流動相為0.1%磷酸溶液-乙腈(梯度洗脫),流速為1.0 ml/min,檢測波長為342 nm。結果:所得腫節風藥材的HPLC指紋圖譜分離度、精密度、重復性和穩定性均良好;10批腫節風藥材指紋圖譜的平均相似度為0.853,不同產地樣品的指紋圖譜差異不大,但4個代表性色譜峰的峰面積有所區別。異嗪皮啶與迷迭香酸的質量濃度均在5~500 mg/ml范圍內與各自峰面積積分值呈良好的線性關系(r分別為0.999 5、0.998 9);平均加樣回收率分別為105.20%、99.52%,RSD分別為1.39%、1.56%(n均為6);10批腫節風藥材中異嗪皮啶與迷迭香酸的含量存在明顯差異,尤以迷迭香酸含量差異較大。結論:該方法簡便、準確、重復性好,可以作為腫節風藥材的質量控制方法。 OBJECTIVE：To study HPLC fingerprints of Sarcandra glabra，and to determine the contents of isofraxidin and rosmarinic acid and establish the method for quality evaluation. METHODS：The samples were separated by Inertsil ODS-4（250 mm× 4.6 mm，5 μm）column with mobile phase consisted of 0.1％ phosphoric acid-acetonitrile（gradient elution）at the flow rate of 1.0 ml/ min. The detection wavelength was set at 342 nm. RESULTS：HPLC fingerprint of S. glabra was established with good separation， precision，repeatability and stability；average similarity of 10 batches of S. glabra was 0.853；there was no significant difference in fingerprint of samples from different producing areas，but the contents of 4 representative peaks were different from one another. The linear ranges of isofraxidin were 5-500 mg/ml（r＝0.999 5）and rosmarinic acid were 5-500 mg/ml（r＝0.998 9）. Average recoveries were 105.20％（RSD＝1.39％ ，n＝6）and 99.52％（RSD＝1.56％ ，n＝6）. The contents of isofraxidin and rosmarinic acid from 10 batches were obviously different from one another，especially rosmarinic acid. CONCLUSIONS：The method is simple，accurate and repeatable，which can be used for the quality control of S. glabra.