Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- India

In the present study, sludge samples were collected from the place where effluents were released by the carpet industries in Bhadohi district. Physico-chemical analysis revealed sludge samples were dark brown in color, pH (6.91 to 9.5), ECe (5.69-11.50 dSm-1), organic C (4.40 - 12.60%), N content (3.50 -13.90%), PO4 content (7.25-13.34%) and K2O content (1.02 -3.21%). Maximum population of bacterial (cfu≈106/g) isolates in sludge samples were found on NA media followed by Kings’ B and NMS. Out of 86 bacterial isolates, 39, 28 and 19 isolates were isolated on NA, Kings’ B and NMS agar media respectively. Sixteen bacterial isolates decolorized the test dyes in respective media plates, 10 decolorized congo red (CR) and 6 decolorized trypan blue (TB) up to higher concentration (25 to 150 ppm) of dyes in agar plate. Pseudomonas sp., Bacillus sp. and Azatobacter sp. and their consortium showed significant decororization of both the dyes after 24h in Nutrient broth. Consortium of C3 (Bacillus sp. + Azatobacter sp.) decolorized 94% of 100 ppm CR and 95% of TB.