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SCN10A Mutation in a Patient with Erythromelalgia Enhances C-Fiber Activity Dependent Slowing

Authors :
Kist, Andreas M.
Sagafos, Dagrun
Rush, Anthony M.
Neacsu, Cristian
Eberhardt, Esther
Schmidt, Roland
Lunden, Lars Kristian
Orstavik, Kristin
Kaluza, Luisa
Meents, Jannis
Zhang, Zhiping
Carr, Thomas Hedley
Salter, Hugh
Malinowsky, David
Wollberg, Patrik
Krupp, Johannes
Kleggetveit, Inge Petter
Schmelz, Martin
Jorum, Ellen
Lampert, Angelika
Namer, Barbara
Kist, Andreas M.
Sagafos, Dagrun
Rush, Anthony M.
Neacsu, Cristian
Eberhardt, Esther
Schmidt, Roland
Lunden, Lars Kristian
Orstavik, Kristin
Kaluza, Luisa
Meents, Jannis
Zhang, Zhiping
Carr, Thomas Hedley
Salter, Hugh
Malinowsky, David
Wollberg, Patrik
Krupp, Johannes
Kleggetveit, Inge Petter
Schmelz, Martin
Jorum, Ellen
Lampert, Angelika
Namer, Barbara
Publication Year :
2016

Abstract

Gain-of-function mutations in the tetrodotoxin (TTX) sensitive voltage-gated sodium channel (Nav) Nav1.7 have been identified as a key mechanism underlying chronic pain in inherited erythromelalgia. Mutations in TTX resistant channels, such as Nav1.8 or Nav1.9, were recently connected with inherited chronic pain syndromes. Here, we investigated the effects of the p.M650K mutation in Nav1.8 in a 53 year old patient with erythromelalgia by micro-neurography and patch-clamp techniques. Recordings of the patient's peripheral nerve fibers showed increased activity dependent slowing (ADS) in CMi and less spontaneous firing compared to a control group of erythromelalgia patients without Nav mutations. To evaluate the impact of the p. M650K mutation on neuronal firing and channel gating, we performed current and voltage-clamp recordings on transfected sensory neurons (DRGs) and neuroblastoma cells. The p. M650K mutation shifted steady-state fast inactivation of Nav1.8 to more hyperpolarized potentials and did not significantly alter any other tested gating behaviors. The AP half-width was significantly broader and the stimulated action potential firing rate was reduced for M650K transfected DRGs compared to WT. We discuss the potential link between enhanced steady state fast inactivation, broader action potential width and the potential physiological consequences.

Details

Database :
OAIster
Notes :
application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1233916732
Document Type :
Electronic Resource
Full Text :
https://doi.org/10.1371.journal.pone.0161789