High throughput droplet sorting of yeast for p-Coumaric acid production detected by co-encapsulated E. coli biosensor bacteria

We present the optimization of E. coli biosensor bacteria and demonstrate their use to detect and sort co-encapsulated S. cerevisiae yeast cells by their p-Coumaric acid (pCA) production in picoliter microfluidic droplets at high throughput. This strategy allows us to enrich pCA producing cell factories by the cell permeable product pCA using a biosensor in a separate cell.
Conference code: 126047; Export Date: 22 May 2017; Conference Paper; Correspondence Address: Joensson, H.N.; KTH Royal Institute of TechnologySweden; email: hakan.jonsson@biotech.kth.se. QC 20170531