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Current status and future opportunities for serial crystallography at MAX IV Laboratory
- Publication Year :
- 2020
-
Abstract
- Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.
Details
- Database :
- OAIster
- Notes :
- Shilova, Anastasya, Lebrette, Hugo, Aurelius, Oskar, Nan, Jie, Welin, Martin, Kovacic, Rebeka, Ghosh, Swagatha, Safari, Cecilia, Friel, Ross J., Milas, Mirko, Matej, Zdenek, Högbom, Martin, Brändén, Gisela, Kloos, Marco, Shoeman, Robert L., Doak, Bruce, Ursby, Thomas, Håkansson, Maria, Logan, Derek T., Mueller, Uwe
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1235049603
- Document Type :
- Electronic Resource
- Full Text :
- https://doi.org/10.1107.S1600577520008735