Exposure to High-Fat Style Diet Induced Renal and Liver Structural Changes, Lipid Accumulation and Inflammation in Intact and Ovariectomized Female Rats

Yamuna Sucedaram,1 Edward James Johns,2 Ruby Husain,3 Munavvar Abdul Sattar4 ,† Mohammed H Abdulla,2 Giribabu Nelli,3 Nur Syahrina Rahim,5 Manizheh Khalilpourfarshbafi,1 Nor Azizan Abdullah1 1Department of Pharmacology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, 50603, Malaysia; 2Department of Physiology, University College Cork, Cork, T12 K8AF, Ireland; 3Department of Physiology, Universiti Malaya, Kuala Lumpur, 50603, Malaysia; 4School of Pharmaceutical Sciences, Universiti Sains Malaysia, Gelugor, 11800, Pulau Pinang, Malaysia; 5Faculty of Medicine & Health Science, Universiti Sains Islam Malaysia, Nilai, 71800, Malaysia†Prof. Dr. Munavvar Abdul Sattar passed away on January 6, 2021Correspondence: Nor Azizan AbdullahDepartment of Pharmacology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, 50603, MalaysiaTel +60122386655Fax +60379674791Email azizan@ummc.edu.myPurpose: We hypothesized that low estrogen levels aggravate obesity-related complications. Diet-induced obesity can cause distinct pathologies, including impaired glucose tolerance, inflammation, and organ injury that leads to fatty liver and chronic kidney diseases. To test this hypothesis, ovariectomized (OVX) rats were fed a high-fat style diet (HFSD), and we examined structural changes and inflammatory response in the kidney and liver.Methods: Sprague-Dawley female rats were ovariectomized or sham-operated and divided into four groups: sham-operated rats fed a normal diet (ND); ovariectomized rats fed a normal diet (OVX-ND); sham-operated rats fed a HFSD; ovariectomized rats fed a high-fat style diet (OVX-HFSD). Mean blood pressure and fasting blood glucose were measured on weeks 0 and 10. The rats were sacrificed 10 weeks after initiation of ND or HFSD, the kidney and liver were harvested for histological, immunohistochemical and immunofluorescence studies.Results: HFSD-fed rats presented a significantly greater adiposity index compared to their ND counter