Effects of Pulsed Electromagnetic Field Intensity on Mesenchymal Stem Cells

Introduction: Bone fractures remain a common injury. Nonunion fractures are often a serious complication where delays in tissue regeneration occur. The use of pulsed electromagnetic fields (PEMFs) for treatment has been studied for years, having reportedly been able to enhance bone regeneration. However, as various PEMF parameters can affect cellular properties differently, it is necessary to adjust each PEMF parameter to achieve the optimal regeneration. Methods: Primary rabbit mesenchymal stem cells (rMSCs) were cultured in vitro in two types of media, namely nondifferentiation and osteogenic differentiation media. The effect of various intensities of PEMF was assessed by evaluating properties such as cellular metabolism, proliferation, and osteogenic differentiation at different time points. Results: The findings suggest that PEMFs had no adverse effect on cellular morphology and mineralization. In contrast, increased metabolic activity was observed at higher PEMF intensity, whereas moderate PEMF intensities had the strongest effect on cell proliferation in both types of culture media. A comparison study was also done between the primary rMSCs against the MC3T3-E1 cells from a previously published article. It was shown that PEMFs improved cell metabolism of MSCs, while maintaining the metabolic activity of MC3T3. Conclusions: PEMFs generally improved cell proliferation for both cell types, whereas leaving cell mineralization unaffected. Taken together, it can be understood that the optimal application of PEMF stimulus, along with the right cell types, is indeed crucial in achieving effective bone regeneration in vitro.
Funding Agencies|Singapore Ministry of EducationMinistry of Education, Singapore [MOE2016-T2-2108]; Agency for Science, Technology and Research (A*STAR) RIE2020 Advanced Manufacturing and Engineering (AME) programmatic grant [A18A8b0059]; Additive Manufacturing for Biological Materials (AMBM) project SP1.1