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Long-term consequences of bilateral cavernous crush injury in normal and diabetic rats: a functional study

Long-term consequences of bilateral cavernous crush injury in normal and diabetic rats: a functional study

Authors :
Castiglione, Fabio
Albersen, Maarten
Fiorenzo, Salvatore
Hedlund, Petter
Cakir, Omer Onur
Pavone, Carlo
Alnajjar, Hussain M.
Joniau, Steven
Muneer, Asif
Castiglione, Fabio
Albersen, Maarten
Fiorenzo, Salvatore
Hedlund, Petter
Cakir, Omer Onur
Pavone, Carlo
Alnajjar, Hussain M.
Joniau, Steven
Muneer, Asif
Publication Year :
2022

Abstract

A recent statement from the European-Society-for-Sexual-Medicine has highlighted the limitations of using the rat model for nerve-sparing prostatectomy. The use of young rats with no comorbidities and the early evaluation of the erectile function (EF) are deemed a source of bias. Our aim was to evaluate the long-term consequences in EF of bilateral nerve cavernous crush- injury (BNCI) in type 1 diabetic (DM) rats 30-male/12-week-old rats were divided into four groups: Sham, BNCI, DM, and BNCI + DM. Sham group underwent an intraperitoneal injection (IP) of saline solution and after 1 month underwent a sham laparotomy. BNCI underwent an IP of saline solution and after 1 month to BNCI. DM underwent an IP of 60 mg/kg-1-streptozotocin (STZ) and after 1 month to a sham laparotomy. BNCI + DM underwent an IP of 60 mg/kg-1-STZ and after 1 month to BNCI. After 5 months from the induction of diabetes, all rats underwent measurement of intracorporeal pressure (ICP) and mean arterial pressure (MAP) during CN-electrostimulation. Multiple groups were compared using Kruskal-Wallis one-way analysis of variance followed by Mann-Whitney U test for post hoc comparisons. Blood glucose-level was higher (p < 0.05) in the groups with DM and BNCI + DM. After 5-months, DM and BNCI + DM also showed a lower weight compared to other groups (p < 0.05). No differences were noted in ICP/MAP between the sham and BNCI. BNCI + DM showed lower ICP/MAP compared to all the groups (p < 0.05). DM Showed lower ICP/MAP compared to Sham and BNCI (p < 0.05). BNCI in rats without comorbidities did not induce long-term erectile dysfunction (ED) suggesting a spontaneous EF recovery. BNCI in DM induced long-term ED. The results of previous short-term studies can only provide evidence on the time to recovery of spontaneous EF as to the actual EF recovery rate.<br />Funding Agencies|European Society for Sexual Medicine (ESSM); European Urological Scholarship Programme (EUSP)

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1280626331
Document Type :
Electronic Resource
Full Text :
https://doi.org/10.1038.s41443-021-00474-z