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New Tailor-Made Alkyl-Aldehyde Bifunctional Supports for Lipase Immobilization

Authors :
Consejo Superior de Investigaciones Científicas (España)
Fundación Ramón Areces
Alnoch, Robson Carlos
Rodrigues de Melo, Ricardo
Palomo, José Miguel
Maltempi de Souza, Emanuel
Krieger, Nadia
Mateo González, César
Consejo Superior de Investigaciones Científicas (España)
Fundación Ramón Areces
Alnoch, Robson Carlos
Rodrigues de Melo, Ricardo
Palomo, José Miguel
Maltempi de Souza, Emanuel
Krieger, Nadia
Mateo González, César
Publication Year :
2016

Abstract

Immobilized and stabilized lipases are important biocatalytic tools. In this paper, different tailor-made bifunctional supports were prepared for the immobilization of a new metagenomic lipase (LipC12). The new supports contained hydrophobic groups (different alkyl groups) to promote interfacial adsorption of the lipase and aldehyde groups to react covalently with the amino groups of side chains of the adsorbed lipase. The best catalyst was 3.5-fold more active and 5000-fold more stable than the soluble enzyme. It was successfully used in the regioselective deacetylation of peracetylated d-glucal. The PEGylated immobilized lipase showed high regioselectivity, producing high yields of the C-3 monodeacetylated product at pH 5.0 and 4 °C.

Details

Database :
OAIster
Publication Type :
Electronic Resource
Accession number :
edsoai.on1306013898
Document Type :
Electronic Resource