Construction of a genetic map telomeric to HAL-A by microsatellite analysis

Authors :: Camaschella, C
Roetto, A
De Sandre, G
Piperno, A
Totaro, A
Dianzani, I
Gasparini, P
Gasparini, P.
PIPERNO, ALBERTO
Camaschella, C
Roetto, A
De Sandre, G
Piperno, A
Totaro, A
Dianzani, I
Gasparini, P
Gasparini, P.
PIPERNO, ALBERTO
Publication Year :: 1993
Abstract: Allele and genotype frquencies of five microsatellites on the short arm of chromosome 6 have been determined in 158 subjects from 14 large Italian families, using polymerase chain reaction amplification and a non-radioactive method of detection. The markers studied were: D6S89, D6S109, D6S105, pLR12 and HLA-F; their heterozygosity ranged from 0.65 to 0.78. Two point and three point linkage analysis among these markers and HLA-A serological types - available for all the studied samples - allowed construction of a partial genetic map telomeric to HLA-A. Our results may be useful for linkage anlaysis of genetic disorders mapping on this region of 6 p.

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