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Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA
- Source :
- Collins , A R , Gedik , C M , Wood , S , White , A , Bubois , J , Duez , P , Rees , J F , Legall , R , Degand , L , Loft , S , Jensen , A , Poulsen , H , Weimann , A , Jensen , B R , Cadet , J , Douki , T , Ravanat , J L , Faure , H , Tripier , M , Morel , I , Sergent , O , Cillard , P , Morin , B , Epe , B , Phoa , N , Hartwig , A , Pelzer , A , Dolara , P , Casalini , C , Guglielmi , F , Luceri , C , Kasai , H , Kido , R , Olinski , R , Bialkowski , K , Duracková , Z , Hlinchikova , L , Korytar , P , Dusinska , M , Mislanova , C , Vina , J , Lloret , A , Möller , L , Hofer , T , Gremaud , E , Fay , L , Stadler , R , Eakins , J , Progna , F , O`Brien , J , Elliott , R , Astley , S B , Bailley , A , Herbert , K , Chauhan , D , Kelly , F , Dunster , C , Lunec , J , Podmore , I , Patel , P , Johnson , S , Evans , M , White , A , Tyrrell , R , Gordon , M , Wild , C & Hardie , L 2002 , ' Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA ' , Free Radical Research , no. vol. 36 , pp. 239-245 .
- Publication Year :
- 2002
-
Abstract
- The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CVof less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.
Details
- Database :
- OAIster
- Journal :
- Collins , A R , Gedik , C M , Wood , S , White , A , Bubois , J , Duez , P , Rees , J F , Legall , R , Degand , L , Loft , S , Jensen , A , Poulsen , H , Weimann , A , Jensen , B R , Cadet , J , Douki , T , Ravanat , J L , Faure , H , Tripier , M , Morel , I , Sergent , O , Cillard , P , Morin , B , Epe , B , Phoa , N , Hartwig , A , Pelzer , A , Dolara , P , Casalini , C , Guglielmi , F , Luceri , C , Kasai , H , Kido , R , Olinski , R , Bialkowski , K , Duracková , Z , Hlinchikova , L , Korytar , P , Dusinska , M , Mislanova , C , Vina , J , Lloret , A , Möller , L , Hofer , T , Gremaud , E , Fay , L , Stadler , R , Eakins , J , Progna , F , O`Brien , J , Elliott , R , Astley , S B , Bailley , A , Herbert , K , Chauhan , D , Kelly , F , Dunster , C , Lunec , J , Podmore , I , Patel , P , Johnson , S , Evans , M , White , A , Tyrrell , R , Gordon , M , Wild , C & Hardie , L 2002 , ' Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA ' , Free Radical Research , no. vol. 36 , pp. 239-245 .
- Notes :
- English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1322510273
- Document Type :
- Electronic Resource