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The hCOMET project:International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders

Authors :
Mili, Mirta
Ceppi, Marcello
Bruzzone, Marco
Azqueta, Amaya
Brunborg, Gunnar
Godschalk, Roger
Koppen, Gudrun
Langie, Sabine
Møller, Peter
Teixeira, Joao Paulo
Alija, Avdulla
Anderson, Diana
Andrade, Vanessa
Andreoli, Cristina
Asllani, Fisnik
Bangkoglu, Ezgi Eyluel
Barancokova, Magdalena
Basaran, Nursen
Boutet-Robinet, Elisa
Buschini, Annamaria
Cavallo, Delia
Pereira, Cristiana Costa
Costa, Carla
Costa, Solange
Silva, Juliana Da
Bo, Cristian Del
Sreckovic, Vesna Dimitrijevic
Djelic, Ninoslav
Durackova, Zdenka
Dvorakova, Monika
Gajski, Goran
Galati, Serena
Lima, Omar Garcia
Giovannelli, Lisa
Goroshinskaya, Irina A.
Grindel, Annemarie
Gutzkow, Kristine B.
Hernandez, Alba
Hernandez, Carlos
Holven, Kirsten B.
Ibero-Baraibar, Idoia
Ottestad, Inger
Kadioglu, Ela
Kazimirova, Alena
Kuznetsova, Elena
Ladeira, Carina
Laffon, Blanca
Lamonaca, Palma
Lebailly, Pierre
Louro, Henriqueta
Cardoso, Tania Mandina
Marcon, Francesca
Marcos, Ricard
Moretti, Massimo
Moretti, Silvia
Najafzadeh, Mojgan
Nemeth, Zsuzsanna
Neri, Monica
Novotna, Bozena
Orlow, Irene
Paduchova, Zuzana
Pastor, Susana
Perdry, Herve
Spremo-Potparevic, Biljana
Ramadhani, Dwi
Riso, Patrizia
Rohr, Paula
Rojas, Emilio
Rossner, Pavel
Safar, Anna
Sardas, Semra
Silva, Maria Joao
Sirota, Nikolay
Smolkova, Bozena
Staruchova, Marta
Stetina, Rudolf
Stopper, Helga
Surikova, Ekaterina I.
Ulven, Stine M.
Ursini, Cinzia Lucia
Valdiglesias, Vanessa
Valverde, Mahara
Vodicka, Pavel
Volkovova, Katarina
Wagner, Karl-Heinz
Zivkovic, Lada
Dusinska, Maria
Collins, Andrew R.
Bonassi, Stefano
Mili, Mirta
Ceppi, Marcello
Bruzzone, Marco
Azqueta, Amaya
Brunborg, Gunnar
Godschalk, Roger
Koppen, Gudrun
Langie, Sabine
Møller, Peter
Teixeira, Joao Paulo
Alija, Avdulla
Anderson, Diana
Andrade, Vanessa
Andreoli, Cristina
Asllani, Fisnik
Bangkoglu, Ezgi Eyluel
Barancokova, Magdalena
Basaran, Nursen
Boutet-Robinet, Elisa
Buschini, Annamaria
Cavallo, Delia
Pereira, Cristiana Costa
Costa, Carla
Costa, Solange
Silva, Juliana Da
Bo, Cristian Del
Sreckovic, Vesna Dimitrijevic
Djelic, Ninoslav
Durackova, Zdenka
Dvorakova, Monika
Gajski, Goran
Galati, Serena
Lima, Omar Garcia
Giovannelli, Lisa
Goroshinskaya, Irina A.
Grindel, Annemarie
Gutzkow, Kristine B.
Hernandez, Alba
Hernandez, Carlos
Holven, Kirsten B.
Ibero-Baraibar, Idoia
Ottestad, Inger
Kadioglu, Ela
Kazimirova, Alena
Kuznetsova, Elena
Ladeira, Carina
Laffon, Blanca
Lamonaca, Palma
Lebailly, Pierre
Louro, Henriqueta
Cardoso, Tania Mandina
Marcon, Francesca
Marcos, Ricard
Moretti, Massimo
Moretti, Silvia
Najafzadeh, Mojgan
Nemeth, Zsuzsanna
Neri, Monica
Novotna, Bozena
Orlow, Irene
Paduchova, Zuzana
Pastor, Susana
Perdry, Herve
Spremo-Potparevic, Biljana
Ramadhani, Dwi
Riso, Patrizia
Rohr, Paula
Rojas, Emilio
Rossner, Pavel
Safar, Anna
Sardas, Semra
Silva, Maria Joao
Sirota, Nikolay
Smolkova, Bozena
Staruchova, Marta
Stetina, Rudolf
Stopper, Helga
Surikova, Ekaterina I.
Ulven, Stine M.
Ursini, Cinzia Lucia
Valdiglesias, Vanessa
Valverde, Mahara
Vodicka, Pavel
Volkovova, Katarina
Wagner, Karl-Heinz
Zivkovic, Lada
Dusinska, Maria
Collins, Andrew R.
Bonassi, Stefano
Source :
Mili , M , Ceppi , M , Bruzzone , M , Azqueta , A , Brunborg , G , Godschalk , R , Koppen , G , Langie , S , Møller , P , Teixeira , J P , Alija , A , Anderson , D , Andrade , V , Andreoli , C , Asllani , F , Bangkoglu , E E , Barancokova , M , Basaran , N , Boutet-Robinet , E , Buschini , A , Cavallo , D , Pereira , C C , Costa , C , Costa , S , Silva , J D , Bo , C D , Sreckovic , V D , Djelic , N , Durackova , Z , Dvorakova , M , Gajski , G , Galati , S , Lima , O G , Giovannelli , L , Goroshinskaya , I A , Grindel , A , Gutzkow , K B , Hernandez , A , Hernandez , C , Holven , K B , Ibero-Baraibar , I , Ottestad , I , Kadioglu , E , Kazimirova , A , Kuznetsova , E , Ladeira , C , Laffon , B , Lamonaca , P , Lebailly , P , Louro , H , Cardoso , T M , Marcon , F , Marcos , R , Moretti , M , Moretti , S , Najafzadeh , M , Nemeth , Z , Neri , M , Novotna , B , Orlow , I , Paduchova , Z , Pastor , S , Perdry , H , Spremo-Potparevic , B , Ramadhani , D , Riso , P , Rohr , P , Rojas , E , Rossner , P , Safar , A , Sardas , S , Silva , M J , Sirota , N , Smolkova , B , Staruchova , M , Stetina , R , Stopper , H , Surikova , E I , Ulven , S M , Ursini , C L , Valdiglesias , V , Valverde , M , Vodicka , P , Volkovova , K , Wagner , K-H , Zivkovic , L , Dusinska , M , Collins , A R & Bonassi , S 2021 , ' The hCOMET project : International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders ' , Mutation Research - Reviews , vol. 787 , 108371 .
Publication Year :
2021

Abstract

The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project a COST Action launched in 2016 represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.(c) 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creative

Details

Database :
OAIster
Journal :
Mili , M , Ceppi , M , Bruzzone , M , Azqueta , A , Brunborg , G , Godschalk , R , Koppen , G , Langie , S , Møller , P , Teixeira , J P , Alija , A , Anderson , D , Andrade , V , Andreoli , C , Asllani , F , Bangkoglu , E E , Barancokova , M , Basaran , N , Boutet-Robinet , E , Buschini , A , Cavallo , D , Pereira , C C , Costa , C , Costa , S , Silva , J D , Bo , C D , Sreckovic , V D , Djelic , N , Durackova , Z , Dvorakova , M , Gajski , G , Galati , S , Lima , O G , Giovannelli , L , Goroshinskaya , I A , Grindel , A , Gutzkow , K B , Hernandez , A , Hernandez , C , Holven , K B , Ibero-Baraibar , I , Ottestad , I , Kadioglu , E , Kazimirova , A , Kuznetsova , E , Ladeira , C , Laffon , B , Lamonaca , P , Lebailly , P , Louro , H , Cardoso , T M , Marcon , F , Marcos , R , Moretti , M , Moretti , S , Najafzadeh , M , Nemeth , Z , Neri , M , Novotna , B , Orlow , I , Paduchova , Z , Pastor , S , Perdry , H , Spremo-Potparevic , B , Ramadhani , D , Riso , P , Rohr , P , Rojas , E , Rossner , P , Safar , A , Sardas , S , Silva , M J , Sirota , N , Smolkova , B , Staruchova , M , Stetina , R , Stopper , H , Surikova , E I , Ulven , S M , Ursini , C L , Valdiglesias , V , Valverde , M , Vodicka , P , Volkovova , K , Wagner , K-H , Zivkovic , L , Dusinska , M , Collins , A R & Bonassi , S 2021 , ' The hCOMET project : International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders ' , Mutation Research - Reviews , vol. 787 , 108371 .
Notes :
application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1322762833
Document Type :
Electronic Resource