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Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery
- Source :
- Panou , D A , Diedrichsen , R G , Kristensen , M & Nielsen , H M 2022 , Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery . in Ü Langel (ed.) , Cell Penetrating Peptides : Methods and Protocols . Springer , Methods in Molecular Biology , vol. 2383 , pp. 371-384 .
- Publication Year :
- 2022
-
Abstract
- This chapter describes the use of cell-penetrating peptides (CPPs) as carriers for transepithelial delivery of therapeutic peptides. Assessment of transepithelial peptide permeation and the mechanisms of action that permeability enhancing drug carriers exert on the epithelium requires subtle sample preparation and analysis by orthogonal methods. Here, the preparation and use of CPP-insulin physical mixture samples including the quantification of insulin by enzyme-linked immunosorbent assay (ELISA) is described. In addition, effects of CPPs on the epithelium and its barrier properties immediately upon exposure and after a recovery period are evaluated by epithelial cell viability, transepithelial electrical resistance, immunostaining of the tight junction associated zonula occludens (ZO-1) protein, and actin cytoskeleton staining.
Details
- Database :
- OAIster
- Journal :
- Panou , D A , Diedrichsen , R G , Kristensen , M & Nielsen , H M 2022 , Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery . in Ü Langel (ed.) , Cell Penetrating Peptides : Methods and Protocols . Springer , Methods in Molecular Biology , vol. 2383 , pp. 371-384 .
- Notes :
- English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1322769110
- Document Type :
- Electronic Resource