Influence of Flax Seeds on the Gut Microbiota of Elderly Patients with Constipation

Jianxia Ma,1,* Jianqin Sun,2,* Huijing Bai,2 Houlian Ma,1 Ke Wang,1 Jun Wang,1 Xiaofeng Yu,1 Yiru Pan,3 Jianfeng Yao1 1Department of Gastroenterology, Huadong Hospital Affiliated to Fudan University, Shanghai, People’s Republic of China; 2Clinical Nutrition Center, Huadong Hospital Affiliated to Fudan University, Shanghai, People’s Republic of China; 3Clinical Laboratory, Huadong Hospital Affiliated to Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jianfeng Yao, Department of Gastroenterology, Huadong Hospital Affiliated to Fudan University, No. 221. Road West Yanan, Shanghai, 200040, People’s Republic of China, Tel +862162483180, Email yjf1966@126.comPurpose: This study was to explore the influence of flax seeds on the gut microbiota of elderly patients with functional constipation.Patients and Methods: Sixty elderly patients (68.68± 8.73 years) with functional constipation were recruited between January 2018 and March 2018. They received oral flax seeds (50 g/d) for one month. Bowel habits and adverse events were recorded before and after treatment. Fresh stool was collected before and after treatment and the amplification product of 16S rRNA V5 region was sequenced using the next-generation sequencing technique on the Ion Torrent PGM platform. The gut microbiota were analyzed before and after flax seeds treatment in the same subject.Results: Flax-seed treatment significantly increased the frequency of defecation and decreased abdominal distension in elderly patients with chronic constipation. The majority of gut bacteria belonged to the phyla of Firmicutes, Bacteroidetes, and Proteobacteria, accounting for 98.71%. After flax seeds treatment, the diversity of bacterial clusters significantly increased with increases of Roseburia_hominis, Pseudomonas_azotoformans, uncultured_Clostridiales_bacterium, Blautia_obeum, Ruminococcus_sp._16442, Pyramidobacter_piscolens