Assessing the aquatic toxicity of Insensitive Munitions (IM) compounds using 10-day aqueous exposures with the amphipod Hyalella azteca

ERDC/EL SR-18-5 Environmental Quality and Technology (EQT) Research Program Assessing the Aquatic Toxicity of Insensitive Munitions (IM) Compounds Using 10-Day Aqueous Exposures with the Amphipod Hyalella azteca Scientific Operating Procedure Series: Characterization of IMX Ecotoxicological Effects Environmental Laboratory Guilherme R. Lotufo, Jacob K. Stanley, and Mark L. Ballentine July 2018 Approved for public release; distribution is unlimited. The U.S. Army Engineer Research and Development Center (ERDC) solves the nation’s toughest engineering and environmental challenges. ERDC develops innovative solutions in civil and military engineering, geospatial sciences, water resources, and environmental sciences for the Army, the Department of Defense, civilian agencies, and our nation’s public good. Find out more at www.erdc.usace.army.mil. To search for other technical reports published by ERDC, visit the ERDC online library at http://acwc.sdp.sirsi.net/client/default. Environmental Quality and Technology (EQT) Research Program ERDC/EL SR-18-5 July 2018 Assessing the Aquatic Toxicity of Insensitive Munitions (IM) Compounds Using 10-day Aqueous Exposures with the Amphipod Hyalella azteca Guilherme R. Lotufo, and Mark L. Ballentine U.S. Army Engineer Research and Development Center (ERDC) Environmental Laboratory (EL) 3909 Halls Ferry Road Vicksburg, MS 39180-6199 Jacob K. Stanley Stanley Environmental Consulting Waynesboro, MS 39367 Final Report Approved for public release; distribution is unlimited. Prepared for Headquarters, U.S. Army Corps of Engineers Washington, DC 20314-1000 Under Project 398708, “Environmental Fate in the Life Cycle Analysis of New Materials” ERDC/EL SR-18-5 ii Abstract This scientific operating procedure (SOP) provides a standard method for assessing the aquatic toxicity of insensitive munitions (IM) compounds using the freshwater amphipod Hyalella azteca. The endpoints for the 10-day static-renewal test are survival and growth. The 10-day t