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Oxidative Stress and Lipid Dysregulation in Lipid Droplets : A Connection to Chronic Kidney Disease Revealed in Human Kidney Cells

Authors :
1000060802634
Chen, Zhen
Shrestha, Rojeet
Yang, Xiaoyue
Wu, Xunzhi
Jia, Jiaping
1000070197622
Chiba, Hitoshi
1000090337030
Hui, Shu-Ping
1000060802634
Chen, Zhen
Shrestha, Rojeet
Yang, Xiaoyue
Wu, Xunzhi
Jia, Jiaping
1000070197622
Chiba, Hitoshi
1000090337030
Hui, Shu-Ping
Publication Year :
2022

Abstract

Chronic kidney disease (CKD), which is defined as a condition causing the gradual loss of kidney function, shows renal lipid droplet (LD) accumulation that is associated with oxidative damage. There is a possibility that an LD abnormality in quality plays a role in CKD development. This study aimed to explore the chemical composition of LDs that are induced in human kidney cells during exposure to free fatty acids as an LD source and oxidized lipoproteins as oxidative stress. The LDs were aspirated directly from cells using nanotips, followed by in-tip microextraction, and the LD lipidomic profiling was conducted using nanoelectrospray mass spectrometry. As a result, the free fatty acids increased the LD lipid content and, at the same time, changed their composition significantly. The oxidized lipoproteins caused distorted proportions of intact lipids, such as triacylglycerols (TG), phosphatidylcholines (PC), phosphatidylethanolamines (PE), and cholesteryl esters (CE). Notably, the oxidized lipids, including the hydroperoxides of TG, PC, and PE, exhibited significant elevations in dose-dependent manners. Furthermore, the dysregulation of intact lipids was paralleled with the accumulation of lipid hydroperoxides. The present study has revealed that the oxidation of lipids and the dysregulation of the lipid metabolism coexisted in LDs in the kidney cells, which has provided a potential new target for diagnosis and new insights into CKD.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1375181154
Document Type :
Electronic Resource