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Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology

Authors :
Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Ministerio de Economía y Competitividad (MINECO). España
Montero Lobato, Zaida
Ramos-Merchante, Adrián
Fuentes, Juan Luis
Sayago, Ana
Fernández Recamales, Ángeles
Martínez Espinosa, Rosa María
Vega Piqueres, José María
Vílchez, Carlos
Garbayo, Inés
Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Ministerio de Economía y Competitividad (MINECO). España
Montero Lobato, Zaida
Ramos-Merchante, Adrián
Fuentes, Juan Luis
Sayago, Ana
Fernández Recamales, Ángeles
Martínez Espinosa, Rosa María
Vega Piqueres, José María
Vílchez, Carlos
Garbayo, Inés
Publication Year :
2018

Abstract

Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81 °C, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 ± 0.64) and production of total carotenoids (3.34 ± 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 ± 0.98) and carotenoid production (3.74 ± 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1416304747
Document Type :
Electronic Resource