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Delivery of E. coli Nissle to the mouse gut by mucoadhesive microcontainers does not improve its competitive ability against strains linked to ulcerative colitis

Authors :
Bondegaard, Pi Westi
Torp, Anders Meyer
Guerra, Priscila
Kristensen, Katja Ann
Christfort, Juliane Fjelrad
Krogfelt, Karen Angeliki
Nielsen, Line Hagner
Zor, Kinga
Boisen, Anja
Mortensen, Martin Steen
Bahl, Martin Iain
Licht, Tine Rask
Bondegaard, Pi Westi
Torp, Anders Meyer
Guerra, Priscila
Kristensen, Katja Ann
Christfort, Juliane Fjelrad
Krogfelt, Karen Angeliki
Nielsen, Line Hagner
Zor, Kinga
Boisen, Anja
Mortensen, Martin Steen
Bahl, Martin Iain
Licht, Tine Rask
Source :
Bondegaard , P W , Torp , A M , Guerra , P , Kristensen , K A , Christfort , J F , Krogfelt , K A , Nielsen , L H , Zor , K , Boisen , A , Mortensen , M S , Bahl , M I & Licht , T R 2023 , ' Delivery of E. coli Nissle to the mouse gut by mucoadhesive microcontainers does not improve its competitive ability against strains linked to ulcerative colitis ' , FEMS Microbiology Letters , vol. 370 , fnad110 .
Publication Year :
2023

Abstract

For patients with ulcerative colitis (UC), administration of the probiotic E. coli Nissle (EcN) holds promise for alleviation of disease symptoms. The mechanisms are unclear, but it has been hypothesised that a capacity of the probiotic to outcompete potentially detrimental UC-associated E. coli strains plays an important role. However, this could previously not be confirmed in a mouse model of competition between EcN and two UC-associated strains, as reported by Petersen et al. 2011. In the present study, we re-evaluated the idea, hypothesising that delivery of EcN by a micro device dosing system (microcontainers), designed for delivery into the intestinal mucus, could support colonisation and confer a competition advantage compared to classical oral dosing. Six groups of mice were pre-colonised with one of two UC-associated E. coli strains followed by oral delivery of EcN, either in capsules containing microcontainers with freeze-dried EcN powder, capsules containing freeze-dried EcN powder, or as a fresh sucrose suspension. Co-colonisation between the probiotic and the disease-associated strains was observed regardless of dosing method, and no competition advantages linked to microcontainer delivery were identified within this setup. Other approaches are thus needed if the competitive capacity of EcN in the gut should be improved.

Details

Database :
OAIster
Journal :
Bondegaard , P W , Torp , A M , Guerra , P , Kristensen , K A , Christfort , J F , Krogfelt , K A , Nielsen , L H , Zor , K , Boisen , A , Mortensen , M S , Bahl , M I & Licht , T R 2023 , ' Delivery of E. coli Nissle to the mouse gut by mucoadhesive microcontainers does not improve its competitive ability against strains linked to ulcerative colitis ' , FEMS Microbiology Letters , vol. 370 , fnad110 .
Notes :
application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1426749752
Document Type :
Electronic Resource