Y-STR genetic diversity in autochthonous Andalusians from Huelva and Granada provinces (Spain)

Financial support has been provided by the Spanish Ministry of Science and Innovation: Research Projects: CGL2010-15191/BOS, and CGL2006-04749/BOS (to RC) and by the Italian Ministero dell’Istruzione, dell’Universita` e della Ricerca: MIURPRIN 2007 (to AN).
Seventeen Y-chromosomal short tandem repeats (STRs) were analyzed in 347 healthy, unrelated, autochthonous males from the Andalusian provinces of Huelva (N=167) and Granada (N=180). AmpFlSTR Y-filer PCR Amplification kit (Applied Biosystems) was used to type the Y-STR markers. A total of 156 and 166 different haplotypes for the 17 Y-STR set were detected in Huelva, and Granada, respectively. The same haplotype diversity was found for both samples (0.998±0.001), and the overall discrimination capacity was 0.904. The most common minimal haplotype (DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393) in both subpopulations was 14-13-16-24-11-13-13, which is also the most frequent haplotype among Atlantic European populations. Comparison analysis using pairwise R(ST) values and Analysis of Molecular Variance (AMOVA) revealed a significant genetic distance between our Andalusian samples and other ones from the northern Iberian fringe (including Basque and Pyrenean populations). However, results from the multi-dimensional scaling analysis (MDS) yielded a well-defined group of Iberian populations separated from the other Mediterranean clusters observed.
Ministerio de Ciencia e Innovación
Depto. de Biodiversidad, Ecología y Evolución
Fac. de Ciencias Biológicas
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