Carrageenan-fed and control microbiota DataSet

The aim of this work was to determine the possible impact of carrageenan on intestinal microbiome, and the impact of this carrageenan -fed microbiome on the intestinal barrier function. Thus, carrageenan was added at increasing doses (in the range 0.3-3 g/L) 3 times daily, for one week for each dose, to a pool of stabilized infant faecal microbiota under stable conditions in the BFBL simulator of the colonic ecosystem. Samples were collected daily from the three colonic reactors. The microbiota composition was analysed by 16S rDNA amplicon-based metagenomics. The impact of this additive on the intestinal barrier function was approached by measurement of Transepithelial Electrical Resistance, the Lucifer Yellow paracellular flux through the Caco-2 cells and the expressions of tight junctions’ proteins.