Carrageenan-fed and control mice microbiota DataSet

The objective of this study was to test the effects of CGN consumption on the gut microbiota and the intestinal homeostasis of male and female young mice. Female and male ICR-CD1 mice (8 weeks old) received orally 540 mg/kg/day of CGN. Fecal material was analyzed to describe changes in the fecal microbiota, based on the analysis of bacterial 16S rRNA gene (V3-V4 region) amplicon sequences. Non-significant microbiota taxonomical changes associated to CGN intake were obtained in the mice stools, resulting the housing time in an increase of bacterial groups belonging to the Bacteroidota phylum. The PICRUSt2 functional predictions based on 16S rDNA amplicons showed an overall increase in functional clusters of orthologous genes (COG) involved in carbohydrate transport and metabolism. A significant increase in citotoxicity of fecal supernatants was observed in CGN-fed mice.