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Heteroatom-tagged proteomics of lung cancer and chronic obstructive pulmonary disease human serum reveal alterations in selenoproteins

Heteroatom-tagged proteomics of lung cancer and chronic obstructive pulmonary disease human serum reveal alterations in selenoproteins

Authors :
Junta de Andalucía
Sociedad Española de Neumología y Cirugía Torácica
Fundación Neumosur
Instituto de Salud Carlos III
Grupo Menarini
AstraZeneca
Universidad de Huelva
Consorcio de Bibliotecas Universitarias de Andalucía
Callejón-Leblic, Belén
Sánchez-Espirilla, Saida
Gotera-Rivera, Carolina
Santana, Rafael
Díaz-Olivares, Isabel
María Marín Trigo, José
Casanova Macario, Ciro
Cosio, Borja G.
Fuster, Antonia
Solanes García, Ingrid
Torres, Juan Pablo de
Feu Collado, Nuria
Cabrera Lopez, Carlos
Amado Diago, Carlos
Romero-Plaza, Amparo
Padrón Fraysse, Luis Alejandro
Márquez Martín, Eduardo
Marín Royo, Margarit
Balcells Vilarnau, Eva
Llunell Casanovas, Antonia
Martínez-González, Cristina
Bautista Galdíz Iturri, Juan
Lacárcel Bautista, Celia
Gómez-Ariza, José L.
Pereira-Vega, Antonio
Seijo, Luis
López-Campos, J. L.
Peces-Barba, Germán
García-Barrera, Tamara
Junta de Andalucía
Sociedad Española de Neumología y Cirugía Torácica
Fundación Neumosur
Instituto de Salud Carlos III
Grupo Menarini
AstraZeneca
Universidad de Huelva
Consorcio de Bibliotecas Universitarias de Andalucía
Callejón-Leblic, Belén
Sánchez-Espirilla, Saida
Gotera-Rivera, Carolina
Santana, Rafael
Díaz-Olivares, Isabel
María Marín Trigo, José
Casanova Macario, Ciro
Cosio, Borja G.
Fuster, Antonia
Solanes García, Ingrid
Torres, Juan Pablo de
Feu Collado, Nuria
Cabrera Lopez, Carlos
Amado Diago, Carlos
Romero-Plaza, Amparo
Padrón Fraysse, Luis Alejandro
Márquez Martín, Eduardo
Marín Royo, Margarit
Balcells Vilarnau, Eva
Llunell Casanovas, Antonia
Martínez-González, Cristina
Bautista Galdíz Iturri, Juan
Lacárcel Bautista, Celia
Gómez-Ariza, José L.
Pereira-Vega, Antonio
Seijo, Luis
López-Campos, J. L.
Peces-Barba, Germán
García-Barrera, Tamara
Publication Year :
2024

Abstract

Heteroatom-tagged proteomics allows the absolute quantification of selenoproteins using the heteroatom as a “tag” into a selective and sensitive atomic detector instead of a molecular one. Using this analytical method, about 90% of total selenium in human serum/plasma can be measured as selenoproteins and total selenometabolites and thus, the status of selenium can be determined. Herein, we determined the absolute concentration of selenoproteins in human serum patients with lung cancer (LC) and chronic obstructive pulmonary disease (COPD), a competing cause of morbidity and mortality in smokers as well as an independent risk factor for LC. We conducted an observational study of 154 human serum samples obtained from LC and COPD patients with varying severity of disease, including COPD patients who developed LC during follow-up and healthy controls (HC). Using heteroatom-tagged proteomics, we determined extracellular glutathione peroxidase (eGPx), selenoprotein P (SELENOP), and selenoalbumin (SeAlb). Associations between selenoproteins were also studied as potential biomarkers of disease. The concentration of eGPx was significantly higher in the all-inclusive COPD cohort compared to HC, COPD patients with LC, or those with mild obstructive lung disease, while SELENOP concentration was significantly decreased in LC patients compared to HC and COPD. We found an inverse correlation between SELENOP and SeAlb in HC, but also in LC patients, and especially in patients with COPD and LC. Moreover, we found that eGPx and selenometabolite concentrations were positively associated with LC human serum. Selenoprotein concentrations were altered in COPD and LC when compared to healthy controls suggesting a potential role of the selenoproteome in the diagnosis and/or treatment of these tobacco-related diseases.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1442728666
Document Type :
Electronic Resource