Identification of aqp1ab1 and aqp1ab2 alternative splicing in teleosts [Dataset]

(A-C) Genomic organization of tandemly arranged aqp1ab2 and aqp1ab1 genes in seabream (A) and Atlantic halibut (B), and of the single aqp1ab2 gene in Senegalese sole and Common sole (C). The four exons (1–4) of both genes are represented by blue and red boxes, and the start codons are indicated by upper arrows. Lower arrows indicate the position of the oligonucleotide primers used for RT-PCR. (D-E) Representative RT-PCR analysis of aqp1ab1 and aqp1ab2 expression in different adult tissues from seabream (D) and halibut (E). A single aqp1ab2 mRNA species is expressed in seabream and halibut, whereas two or one splice variants (aqp1ab1_v1 and aqp1ab1_v2) in addition to aqp1ab1 are detected, respectively, as indicated. (F-G) RT-PCR of aqp1ab2 expression in Senegalese sole (F) and Common sole (G) where two splice forms (aqp1ab2_v1 and aqp1ab2 _v2) in addition to aqp1ab2 are amplified in the ovary and testis. In D-G, the sizes (kb) of molecular markers are indicated on the left.