Model-based structure of teleost Aqp1ab1 and Aqp1ab2 and splice variants [Dataset]

(A-C) Schematic diagram of the seabream and halibut aqp1ab1 and sole aqp1ab2 genes, and the different emerging transcript variants identified in each species. The numbers represent the exons, and the initiation and stop codons in each mRNA are indicated. (D-F) Integral membrane views (cartoon renders) of the wild type and variant channels. Canonical transmembrane domains are shown in cyan, loops in magenta and the two conserved Asn-Pro-Ala (NPA) motifs in dark blue. Variant regions are shown in red. In sole Aqp1ab2_v2 (C and F), the splicing of the distal region of intron 2 causes out-of-frame readthrough of exon 3 and the spliced exon 4 resulting in a premature stop codon and the truncated protein.