1. Plasmonic ZnO nanorods/Au substrates for protein microarrays with high sensitivity and broad dynamic range.
- Author
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Liu, Chang, Meng, Fanling, Zheng, Weitao, Xue, Tianyu, Jin, Zhao, Wang, Zhenxin, and Cui, Xiaoqiang
- Subjects
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PLASMONICS , *ZINC oxide , *NANORODS , *GOLD compounds , *SUBSTRATES (Materials science) - Abstract
Microarrays require high sensitivity and a broad dynamic range for high-throughput diagnostics and proteomic analysis. We present the fabrication of novel plasmonic protein microarrays using nanostructured ZnO-Nanorods (ZnO-NRs) on 50 nm Au film that exhibits an enhancement of fluorescence up to 200-fold. The as-prepared plasmonic protein microarrays were used for the detection of the carcinoembryonic antigen (CEA) cancer biomarker. The plasmonic enhancement resulted in a detection limit of 27 pg mL −1 in 0.01 M PBS and a dynamic range of 100 pg mL −1 to 100 μg mL −1 . The ZnO-NRs/Au substrates can be mass-manufactured, which is highly promising for the development of low cost, sensitive, and high-throughput protein assay platform for applications in clinical diagnosis. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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