1. In vivo cellular MRI of dendritic cell migration using micrometer-sized iron oxide (MPIO) particles.
- Author
-
Rohani, Roja, Chickera, Sonali, Willert, Christy, Chen, Yuhua, Dekaban, Gregory, Foster, Paula, de Chickera, Sonali N, Dekaban, Gregory A, and Foster, Paula J
- Subjects
- *
IRON oxides , *DENDRITIC cells , *MAGNETIC resonance imaging , *HISTOLOGY , *CELLS , *FLUORESCENCE microscopy , *ANIMAL experimentation , *APOPTOSIS , *BONE marrow , *CELL physiology , *CELL motility , *COMPARATIVE studies , *IRON compounds , *LIGHT , *LYMPH nodes , *RESEARCH methodology , *MEDICAL cooperation , *MICE , *ORGANIC compounds , *PARTICLES , *RESEARCH , *STAINS & staining (Microscopy) , *PHENOTYPES , *EVALUATION research - Abstract
Purpose: This study seeks to assess the use of labeling with micron-sized iron oxide (MPIO) particles for the detection and quantification of the migration of dendritic cells (DCs) using cellular magnetic resonance imaging (MRI).Procedures: DCs were labeled with red fluorescent MPIO particles for detection by cellular MRI and a green fluorescent membrane dye (PKH67) for histological detection. MPIO-labeled DCs or unlabeled control DCs were injected into mice footpads at two doses (0.1 × 10(6) and 1 × 10(6)). Images were acquired at 3 Tesla before DC injection and 2, 3, and 7 days post-DC injection.Results: Labeling DCs with MPIO particles did not affect viability, but it did alter markers of DC activation and maturation. MRI and fluorescence microscopy allowed for the detection of MPIO-labeled DCs within the draining popliteal nodes after their injection into the footpad.Conclusions: This paper presents the first report of the successful use of fluorescent MPIO particles to label and track DC migration. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF