1. Rapid detection of Bombyx mori bidensovirus by loop-mediated isothermal amplification based lateral flow dipstick assay for field applications.
- Author
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Lü, Peng, He, Yaling, Lin, Feng, Pan, Ye, Yu, Qian, Guo, Zhongjian, Li, Chengjun, Wang, Huiying, Yao, Qin, Fu, Yan, and Chen, Keping
- Subjects
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SILKWORMS , *GROUNDWATER flow , *SINGLE-stranded DNA , *INSECT viruses , *DETECTION limit , *FECES - Abstract
• A rapid method for BmBDV detection in the field was developed. • The LAMP-LFD method requires only simple faeces-heating and is completed in 45 min. • The method can be adapted for rapid detection of other silkworm pathogens. Bombyx mori bidensovirus (BmBDV), is the only bipartite single-stranded DNA (ssDNA) insect virus reported to date. BmBDV causes fatal flacherie disease in silkworm, resulting in large economic losses to sericulture. We developed a loop-mediated isothermal amplification with lateral flow dipstick (LAMP-LFD) method that can successfully and rapidly detect BmBDV DNA with a low limit of detection (5 fg, 400 copies of the BmBDV genome). The method was evaluated and improved for direct field diagnosis using silkworm faeces. In the field, the actual limit of detection was ∼50 fg (4000 copies of the BmBDV genome). The results demonstrated that, compared with traditional methods for BmBDV detection, our new LAMP-LFD method was much more rapid, sensitive and cost-effective, with less dependence on equipment, making it easier to use. The method has potential to be translated into a new diagnostic product for field applications in the sericulture industry. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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