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In order to prepare high-performance and low-cost electrode materials, nickel ion-doped polyANI-coated biomass carbon composite materials (Ni@ PANI/BC) were synthesized by chemical oxidation polymerization using August melon peel biomass carbon (BC) as the matrix, and nickel nitrate and aniline as raw materials. The structures of the Ni@ PANI/BC composites were characterized by SEM, EDS, X-ray photoelectron energy spectrum (XPS), thermal weight analysis (TG) and specific surface area test (BET), and the electrochemical properties of the composites were measured by cyclic voltammetry (CV), constant current charge and discharge (GCD) test and electrochemical impedance spectroscopy (EIS). The effects of nickel ion doping on the structure and electrochemical properties of Ni@ PANI/BC were investigated. It is shown that nickel ion doped BC forms a porous cross-linked mesh porous structure with large specific surface area and good electrical conductivity. When the mass ratio of nickel hydrate nitrate, aniline and BC is 1. 5∶5∶3, the obtained electrode material has the best electrochemical performance. At 1 A/g current density, Ni@ PANI/BC mass capacitance is up to 608. 4 F/g, 5 000 cycles with a capacity retention rate of 85. 4%, which demonstrates Ni@ PANI/BC materials have electrochemical properties comparable to the relevant materials reported in the literature. [ABSTRACT FROM AUTHOR]

目的, 观察依达拉奉右莰醇静脉滴注治疗急性动脉粥样硬化性脑梗死 (ACI)的临床效果, 并探讨其治疗 作用机制。方法, 选取120例ACI患者, 随机均分为实验组和对照组。两组均给予常规治疗, 实验组在常规治疗的 基础上, 加用依达拉奉右莰醇注射用浓溶液 15 mL, 将其溶于生理盐水100 mL中稀释后静脉滴注, 30 min内滴注完 毕, 每天2次；两组疗程均14 d。比较两组总有效率。治疗前和治疗14天, 对患者进行NIHSS评分 (评价神经系统缺 损程度)；治疗90 d, 对患者进行改良Rankin量表评分 (mRS评分, 评价独立生活能力), 计算mRS评分≤1分的患者比 例。治疗前和治疗14天, 采用ELISA方法检测两组的人甲壳质酶蛋白40 (YKL-40)、人可溶性CD40 (sCD40)。统计 两组治疗期间的不良反应发生率。结果, 实验组总有效率高于对照组 (P<0. 05)。与治疗前比较, 治疗后两组 NIHSS评分均降低, 以实验组为著 (P均<0. 05)。实验组治疗后90 d的mRS评分≤1分的患者比例高于对照组 (P均< 0. 05)。与治疗前比较, 治疗后两组YKL-40、sCD40水平均降低, 以实验组为著 (P均<0. 05)。两组治疗期间均未发 生严重不良反应事件。结论, 依达拉奉右莰醇静脉滴注治疗ACI有效、安全, 可有效改善患者神经功能缺损症状、 提高独立生活能力；依达拉奉右莰醇可能通过降低血清YKL-40、sCD40水平起治疗作用的。 [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical efficacy of combined with fascial sheath suspension (CFS) and shortening of levator (LM) in the treatment of bell-negative severe ptosis. Methods The clinical data of 10 patients (17 eyes) with severe blepharoptosis were reviewed. After cutting the skin along the design line, the tarsus were exposed by separating the orbicularis oculi muscle. The levator palpebrae superioris was separated, subconjunctival swelling anesthetized conjunctiva and Müller muscle, and the levator palpebrae superioris and Müller muscle were peeled off. CFS was found along the upward separation of conjunctiva and Müller muscle, and the CFS and levator palpebrae were fixed in the middle and upper part of the tarsus with a 5-0 silk thread. A suture of the double eyelids was formed. Results Ten patients were followed up for 6 months, and there was no obvious regression or adverse reaction, with beautiful effect and high patient satisfaction. Conclusion CFS suspension combined with LM shortening was used to correct severe ptosis with satisfactory clinical effect and safe operation, which can be popularized and applied in clinical practice. [ABSTRACT FROM AUTHOR]

[Objective] With the proliferation of breeding data brought by new technologies and the new demand for knowledge services in computational breeding, in order to solve the problem of inefficient cross-species subject knowledge acquisition and difficult discovery of elite pleiotropic genes in crop breeding knowledge service. [Method] We constructed trait-regulated-genes ontology model framework, and defined entity hierarchical structure and entity attributes in ontology model. Taking staple crops rice, corn, wheat and model plant Arabidopsis thalliana as data collection objects, a knowledge graph with trait-regulated-genes ontology model as model layer is constructed and experimented. [Result] Finally, the trait-regulated-genes ontology model covering 13 entities, 16 data attributes and 14 object attributes was formed. This model is used as the knowledge graph of ontology layer to realize cross-species subject knowledge association retrieval, mining of elite pleiotropic genes and prediction of gene function across species. [Conclusion] The method of trait-regulated-genes ontology model construction proposed in our study may achieve the correlation discovery of trait regulatory genes across species, improve the efficiency of cross-species subject knowledge acquisition, and support the gene discovery results of multi-dimensional data analysis. This study provides a feasible method path for the mining of pleiotropic genes and gene function prediction, and provides effective data support services for crop breeding scientific research. [ABSTRACT FROM AUTHOR]

Objective To explore the expression of programmed cell death protein 5 in cervical cancer and its relationship with lymph node metastasis. Methods 98 cases of cervical cancer patients admitted to our hospital were selected as the observation group, and 98 cases of cervical benign lesions were selected as the control group. The expression levels of PDCD5 in serum and lesion tissues of the two groups were compared to analyze the relationship between PDCD5 and pathological features of cervical cancer, and the diagnostic value of PDCD5 in lymph node metastasis of patients with cervical cancer was analyzed by ROC curve. Results The expression levels of PDCD5 in serum and lesion tissue of observation group were lower than those of control group (P < 0.05) . With the increase of clinical stage and pathological grade of cervical cancer, the expression of PDCD5 in serum and lesion tissue was significantly decreased (P < 0.05) . Among the 98 patients with cervical cancer, 32 had lymph node metastasis. The expression levels of PDCD5 in serum and lesion tissue of lymph node metastasis group were lower than those of non-lymph node metastasis group (P < 0.05) . ROC curve results showed that the AUCs of PDCD5 in serum and lesion tissue to predict lymph node metastasis of cervical cancer patients were 0.810 and 0.850, respectively, with no statistical significance (P > 0.05) . Conclusion The Programmed cell death protein 5 is closely related to the pathological features of patients with cervical cancer, and it has a good predictive effect on lymph node metastasis, which is worthy of further study and application. [ABSTRACT FROM AUTHOR]

[Objective/Meaning] This paper aimed to comprehensively understand the status quo of the spatial differentiation of hollowing out of rural population in the counties of Fujian Province and its influencing factors. [Methods/Procedures] Based on the data of the seventh population census in Fujian Province, and on the basis of the comprehensive evaluation of the hollowing degree of rural population, the spatial differentiation rule was studied by using the methods such as ESDA-GIS, and then the multiple linear regression model was established to explore the influencing factors. [Results/Conclusions] The results showed that: (1) There were different degrees of hollowness of rural population in the counties of Fujian Province, showing significant regional differences between the coastal and inland areas and between the counties around municipal districts and the counties around non-municipal districts. (2) The degree of hollowness of rural population showed a trend of spatial agglomeration. The highhigh agglomeration areas were mainly distributed in the less developed counties in the inland mountainous area, while the low-low agglomeration areas were mainly distributed in the economically well-developed counties in the coastal areas. (3) Except for the insignificant effect of regional differentiation, the lower the per capita disposable income of rural residents was, the lower the proportion of rural population participating in the subsistence allowances was, the larger the sown area of food crops was and the higher the proportion of educational expenditure in the fiscal expenditure was, the higher the hollowing degree of rural population would be. [ABSTRACT FROM AUTHOR]

Objective To compare the efficacy of radiofrequency thermocoagulation and pulsed radiofrequency for the ganglion impar in treating primary perineal pain. Methods We analyzed 79 patients with primary perineal pain who underwent radiofrequency thermocoagulation (group A) and pulsed radiofrequency (group B) in the ganglion impar from January 2020 to March 2022. VAS, excellent and good rates, sleep quality, postoperative medication usage, complications, and recurrence were evaluated before and 24 h, 1 W, 1 M, 3 M and 6 M after operation. The differences between the two groups were compared. Results The VAS score of group A gradually decreased at each level after operation, and the VAS score of group B gradually increased after 24 hours of operation. The differences between the two groups began to appear 1 week after operation, and the differences further increased with the extension of time (P<0.001). In six months after follow-up, the excellent and good rates of group A (86%) was significantly higher than that of group B (22%). In addition to postoperative perineal skin numbness, group A was superior to group B in improving sleep, postoperative oral medication (pregabalin and opioids), and disease recurrence (P<0.05). Conclusion Radiofrequency thermocoagulation for the ganglion impar can improve the quality of life by reducing pain, improving the excellent and good rates, improving sleep, and reducing recurrence a medication. The effect is better than that of pulsed radiofrequency. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between acidic deoxycholic acid induced mitochondrial DNA (mtDNA) damage and release of human esophageal epithelial cells and cGAS⁃STING pathway in the development of esophageal epithelial cell inflammation. Methods: HEECs were divided into control group and acidic deoxycholic acid treatment group. The viability of cells was measured by CCK ⁃8 assay. Changes of reactive oxygen species, mitochondrial reactive oxygen species and mitochondrial membrane potential were detected by fluorescence microscope and flow cytometry. ATP was detected by the luminometer. The ultrastructure of mitochondria was observed by transmission electron microscope. The mtDNA copy number was evaluated by qPCR. The expressions of γH2AX, cGAS, STING, p⁃NF⁃κB p65 and NF⁃κB p65 were detected by Western blotting. The mRNA expressions of inflammatory cytokines IL⁃6 and IL⁃1β were detected by qPCR. Results: CCK ⁃ 8 assay showed that the viability of cells treated with acidic deoxycholic acid decreased in a dose⁃time dependent manner. The production of intracellular ROS and mtROS increased, while MMP and ATP decreased. Compared with the control group, the expression of γH2AX increased after acidic deoxycholic acid, mtDNA released into the cytoplasm, mtDNA copy number reduced, the expressions of cGAS, STING and p⁃NF⁃κB p65 were increased, and the expressions of inflammatory cytokines IL⁃6 and IL⁃1β were elevated. After pretreatment with cGAS inhibitor RU.521, the expression levels of cGAS and STING were inhibited and the expression of p⁃NF⁃κB p65 was partially inhibited, and the levels of inflammatory cytokines IL⁃6 and IL⁃1β were decreased. Conclusion: The in vitro experiments have shown that acidic deoxycholic acid can induce mitochondrial dysfunction, mitochondrial DNA damage and release, and mediate HEEC inflammation. The mechanism may be related to the activation of cGAS⁃STING pathway. [ABSTRACT FROM AUTHOR]

Objective To provide reference basis for the formulation of medical union association policy by analyzing the influencing factors of the medical staff′s job satisfaction. Methods From July to August 2021,a total of 703 medical staff in 20 medical union units were investigated by using the questionnaire of medical union construction in the Affiliated Hospital of Qinghai University. Logistic regression analysis was used to explore the influencing factors of medical staff′s job satisfaction. Results The overall job satisfaction of the medical union was 57.68%.The job satisfaction of the tertiary hospital was 61.54%,the secondary hospital was 58.64%,and the primary hospital was 53.33%.There were statistically significant differences in the satisfaction of medical staff at different levels of hospitals in continuing medical education and training, teaching rounds and surgical teaching, telemedicine services, and family doctor signing (P＜0.05) .The univariate analysis showed that the main factors affecting the job satisfaction of medical staff in the medical union were age, working years, marital status, staffing and average monthly income (P＜0.05) .The multivariate analysis showed that working years and staffing were the main factors (P＜0.05) .Conclusion In order to promote the development of the medical union, it is suggested to strengthen the publicity work of the medical union, promote the construction of key clinical specialties at the grassroots level by the famous doctor studio, refine the assessment mechanism, and learn from the advanced experience of the regional collaborative service model of active health care services. [ABSTRACT FROM AUTHOR]

Objective To screen and verify a peptide ligand specific for CD44v9. Methods A 12-mer phage peptide library was screened on CD44v9 coated on solid phase. Candidate sequences emerged after sequencing. Candidate phages were selected using enzyme-linked immunosorbent assay. The best sequence was chosen for further study. Binding of C9-3 to CD44v overexpressed HEK-293 cells was determined using immunofluorescence. Binding affinity and specificity were verified on gastric cancer tissues with immunohistochemistry. Results Phages significantly were enriched during panning process. After sequencing, nine individual sequences occurred in 30 selected clones. Among the 9 candidate sequences, C9-3 exhibited the highest frequency. Results of ELISA showed that C9-3 had the highest OD value and selectivity. Thus, C9-3 was chosen for peptide probe synthesis. C9-3 probe stained CD44v overexpressed HEK-293 cells, but not empty vector transfected HEK-293 cells. Immunohistochemistry scores of C9-3 were significantly different between gastric cancer and paracancer tissues (t=3.953, P＜0.01). A linear positive correlation was observed between C9-3 binding and CD44v9 expression (r=0.823, P＜0.01). Conclusion In this study, peptide ligand of CD44v9 was successfully screened. The peptide can bind to cells and cancer tissues via CD44v9. It has potential for gastric targeting probes. [ABSTRACT FROM AUTHOR]

In this paper, the Reflection High Energy Electron Diffraction (RHEED) intensity oscillation is used to measure the homoepitaxial growth of GaAs, and it is found that the growth rate decays according to a certain exponential function relationship with the growth thickness. This attenuation is closely related to the change of the GaAs surface morphology. The increase in the number of surface steps causes the layered growth mode to gradually change from the 2D nucleation mode to the step flow mode. Since the growth rate measured by RHEED intensity oscillation is closely related to the surface roughness, changes in surface conditions will have a certain impact on the growth rate, resulting in a gradual attenuation of the measured growth rate. According to the fitting curve of the attenuation of the growth rate with the increase of the growth thickness, an accurate growth rate can be obtained. [ABSTRACT FROM AUTHOR]

【Objective】The study was conducted to build an evaluation system of modern urban agriculture, measure urban agriculture development level and differences in different areas in Guangdong-Hong Kong-Macao Greater Bay Area (GBA), analyse the driving factors, and put forward the optimization path of the development of urban agriculture in the GBA, providing theoretical support for the high-quality development of urban agriculture in developed urban agglomerations such as the GBA.【Method】The entropy method was used to evaluate the development of modern urban agriculture in the GBA from 2013 to 2020. The Dagum Gini coefficient decomposition was used to analyse the regional differences, and the geographical detector method was used to study the driving factors of urban agriculture development in the GBA. 【Result】The average annual growth rate of modern urban agriculture development level in the GBA is 5.3%, and Guangzhou is the city with the highest development level. The vegetable-basket product support capability in the GBA develops stably, agricultural ecology and sustainable development level rises in volatility, and level of integrated development of three industries develops quickly, with the average annual growth rate of 12.1%. Agglomeration level of advanced agricultural production factors increases slightly, with an average annual growth rate of 0.45%, and modern agricultural management level increases rapidly, with an average annual growth rate of 17.2%. The overall Gini coefficient of the area decline in the volatility, and the differences mainly come from the gap among areas. Currently, proportion of cultivated land area, proportion of three industries and percapita GDP are important driving factors for the development of urban agriculture. The driving factors differ in areas, and the interaction of all factors is “double factor enhancement”, and any two driving factors acting together will enhance the intensity of their effects on urban agriculture differentiation.【Conclusion】During sample observation period, there is a growing trend of the modern urban agriculture in Guangdong-Hong Kong-Macao Greater Bay Area generally, but there are declining trends between the gap of regional development of urban agriculture and the gaps among cities are still large. It is recommended to improve the overall planning and regional shortcomings, the development factors of modern urban agriculture and policy supply. [ABSTRACT FROM AUTHOR]

In order to study the pharmacological effects of the water extract of willow leaf vegetable on anti-hyperglycemia and hyperlipidemia, Wistar rats were induced with type 2 diabetes mellitus by high-fat fructose streptozotocin, and the water extract of willow leaf vegetable was used to test it. After treatment, analyze changes in parameters such as body weight, lipid profile, liver profile, kidney profile, and inflammatory markers. The results showed that the water extract of willow leaf vegetable can cause increased insulin release and decreased fasting blood sugar in rats with type 2 diabetes, and the effect is dose-related. Therefore, the water extract of willow leaf vegetable can maintain the normal functions of liver, kidney and pancreas, and has the effect of lowering blood sugar and blood fat. [ABSTRACT FROM AUTHOR]

Objective • To investigate whether the efficacy of tacrolimus (TAC) monotherapy is non-inferior to TAC combined with glucocorticoids (TAC+GC) in the treatment of idiopathic membranous nephropathy (IMN), and evaluate its safety. Methods • This pilot study was conducted by the Department of Renal Rheumatology and Immunology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine from November 2017 to March 2021. High-risk patients with biopsy-proven IMN were recruited and randomly divided into two groups: TAC monotherapy group only received TAC treatment [an initial dose of 0.050-0.075 mg/(kg・d), maintaining trough blood concentration of 5-10 ng/mL]; TAC combined with GC group (TAC+GC group) received TAC (the same as TAC monotherapy group) combined with prednisone [an initial dose of 0.5 mg/(kg・d) for 8-12 weeks, then tapered until complete withdrawal]. The baseline data of the two groups of patients were collected, and the efficacy indicators and safety indicators of the patients were observed and recorded during the visit. The primary end points of the study were the total remission rate and complete remission rate of proteinuria at 24 weeks of treatment. The secondary end points included the efficacy indicators [24 h urinary protein quantification, serum albumin level and estimated glomerular filtration rate level at 24 weeks of treatment] and the incidence of adverse events (elevated blood glucose, thrombosis and infection). Non-inferiority test was used to compare the total remission rate of proteinuria in the two groups, and the non-inferiority margin was set as 一 10%. Fisher's precision probability test was used to compare the total remission rate, complete remission rate and adverse events rate of the two groups of patients. Non parametric tests were used to compare the differences of the percentage changes of the efficacy indicators from baseline values after treatment between the two groups. Results • A total of 36 IMN patients were enrolled, with 18 in TAC monotherapy group and 18 in TAC+GC group. There was no statistically significant difference between the baseline data of the two groups. During the treatment, 1 case was lost in TAC monotherapy group and 3 cases in TAC+GC group. Finally, 36 patients were included in the full analysis set (FAS), 32 patients were included in the per protocol set (PPS), and 36 patients were included in the safety set. The results of non-inferiority test showed that it was not observed that the efficacy of TAC monotherapy was not inferior to that of TAC combined with GC. The risk difference of total remission rate of proteinuria in the two groups was 0 (95%CI -31.9-31.9, P=0.269), and the lower limit was below the non-inferiority margin. In FAS and PPS, there was no significant difference in complete remission rate and total remission rate between the two groups at 24 weeks of treatment, and no significant difference in the percentage changes of the efficacy indicators from baseline values after treatment between the two groups. Ten patients (4 cases in TAC monotherapy group, and 6 cases in TAC+GC group) developed elevated blood glucose over the period of treatment, with no significant difference between the two groups; one patient (TAC+GC group) developed right upper extremity venous thrombosis. Conclusion* This pilot study finds that the total remission rate and complete remission rate of the two groups are similar at 24 weeks of treatment. The frequency of adverse events in TAC monotherapy group is relatively lower than that in TAC combined with GC group. However, the available data cannot prove that the efficacy of TAC monotherapy is non-inferior to that of TAC combined with GC in the treatment of IMN. [ABSTRACT FROM AUTHOR]

Bifunctional 20V/Ce-Al2O3 （mass percent 20% V2O5 ） catalysts are prepared by simply co-precipitation of 20V2O5/Al2O3 with Ce to improve the acidity of catalyst effectively，and tested for partial oxidation of methanol. The structural properties are characterized by X-ray diffraction（XRD），scanning electron microscopy（SEM-EDS），Raman spectroscopy（Raman），H2 temperature-programmed reduction（H2-TPR） and NH3 temperature-programmed desorption （NH3-TPD） techniques，respectively. The characterization results confirm that the incorporation of Ce in catalyst enhances the surface acidity，which is the key factor to improve the selectivity of DMM，and the interaction between vanadium oxide and the support is enhanced and the redox ability of the catalysts is reduced at the same time. The experimental results show that the catalyst 20V/8CeAl2O3 with mass percent 8% CeO2 exhibits the best catalytic performance and the reaction temperature has a great influence on the methanol oxidation products. Low temperature is more favourable for the formation of DMM. For the catalyst 20V2O5/8Ce-Al2O3，the methanol conversion and the DMM selectivity are 23. 6% and 99. 9% at 170 ℃，respectively. The selectivity of DMM is 99. 9% after 5 runs. The study provides valuable reference for improving the acidity of catalyst. [ABSTRACT FROM AUTHOR]

Objective To unmask the psychological gaps related to suicide ideation in the elderly based on Social Reference Theory, so as to put forward countermeasures for psychological crisis intervention and management. Methods From 2018 to 2019, purposive sampling was adopted to select 19 elderly people with history of suicidal ideation and suicide attempt from 37 geriatric nursing institutions in Ji'nan, Shandong province. Semi-structured interviews were conducted with the elderly, and the collected data were processed by Colaizzi seven-step analysis method. Results The qualitative analysis showed that the elderly with suicidal ideation often experienced strong psychological gaps, including physical condition and function gap, family role gap, socially isolated and lonely gap, filial expectation gap and the gap between the real self and ideal self. Conclusion The psychological gaps serve as the main psychological motivator for suicidal ideation in the elderly, therefore, reducing the psychological gaps can be a new approach for the suicide intervention among the elderly. [ABSTRACT FROM AUTHOR]

Source-separated urine contains a large amount of nutrients, such as nitrogen, phosphorus, and potassium, which can be both resources and pollutants. This article describes the characteristics of the source-separated urine, summarizes the technology for nutrient recovery from the source-separated urine, summarizes the effects of urine on crop growth, and clarifies the main effects of utilization of human urine on soil physicochemical characteristics and drug accumulation. Finally, the prospects for the agricultural utilization of sourceseparated urine as a substitute for chemical fertilizers are identified, which would provide a research direction for the return of human urine as liquid fertilizer. [ABSTRACT FROM AUTHOR]

Objective The flocculent suspended matter in fluid catalytic cracking (FCC) diesel oil from a refinery of CNOOC was separated and analyzed. Its composition, source and possible solution were deduced. Methods A scheme for separating flocculent suspended solids from an oil-water mixture was developed and characterized by Fourier transform ion cyclotron resonance mass spectrometry and Fourier transform ion cyclotron resonance spectrometry. Results The results show that flocculent suspended matter are mainly organic compounds and can be flocculated in a hydrochloric acid environment. The main body of the suspended matter is nitrogen-containing compounds about C40-C55 determined by mass spectrometry, and it shows obvious artificial synthesis characteristics. Conclusions The flocculent suspension is the compound inhibitor of amines and amides or the residue of many kinds of inhibitors. This separation and analysis method can also be used for composition analysis of similar samples, which is helpful for refineries to investigate the causes of similar faults. [ABSTRACT FROM AUTHOR]

Objective To explore and evaluate the accuracy of non-invasive ultrasound cardiac output monitoring technology (USCOM) applied to the monitoring of stroke volume (SV) and cardiac output (CO) in patients of different ages. Methods The patients were divided into youth group (20~<40 years old), middle-aged group (40~<60 years old), and elderly group (60~80 years old) according to their ages. At the same time,each group was given USCOM and transthoracic two-dimensional echocardiography. (TTE) SV and CO were measured, and transthoracic two-dimensional echocardiography was used as the control. Results There was no statistically significant difference between CO and SV measured by TTE and USCOM in the three age stages (P> 0.05). In the youth group, the average difference in SV measured by TTE and USCOM was -2.56 mL, the 95%CI was -14.03-8.92. The average difference in CO measured by TTE and USCOM in the youth group was -0.13 L/min, and the 95% CI was -1.1-0.84.In the middle-aged group, the average difference in SV measured by TTE and USCOM was -3.0 mL, and the 95%CI was -18.49-12.49. In the middle-aged group, the average difference in CO measured by TTE and USCOM was -0.16 L/min, and the 95%CI was -1.31-0.94. The average difference in SV measured by TTE and USCOM in the elderly group was -2.74 mL, and the 95%CI was -19.49-14.55. The average difference of CO measured by TTE and USCOM in the elderly group was -0.18 L/min, and the 95%CI was -1.36-1.00. Conclusion The consistency of SV and CO measured by US- COM and TTE for patients of different age groups is better. USCOM is suitable for hemodynamic monitoring of patients of different age groups. [ABSTRACT FROM AUTHOR]

Objective: To study the anesthetic effect of hydrocodone combined with propofol for painless colonoscopy and to investigate the mechanism of the analgesic effect produced by hydrocodone. Methods: Clean grade male SD rats were randomly divided into four groups: normal group; propofol group; fentanyl combined with propofol group; hydromorphone combined with propofol group. After the model was anesthetized for 10 minutes, colorectal dilation was performed using a fully lubricated balloon for once per 5 min, each lasting 30 s. The abdominal wall withdrawal reflex (AWR3) scores of each group were recorded, and epinephrine and norepinephrine content were detected by ELISA. MAPK p38 expression changes were detected by western blot. Results: (1) 10 minutes after abdominal administration of propofol, the AWR3 scores of the propofol group were significantly higher than those of the fentanyl combined with propofol group and the hydromorphone combined with propofol group (P < 0.000 1, P < 0.000 1. The lowest AWR3 scores were reached 15 minutes after abdominal administration in all three groups. 20 minutes after abdominal administration, the AWR3 scores in the fentanyl combined with propofol group and the hydromorphone combined with propofol group were higher than those in the 10 and 15 minutes, but remained low. (2) The contents of epinephrine and norepinephrine in the fentanyl combined with propofol group and the hydromorphone combined with propofol group decreased significantly compared with those in the propofol group (epinephrine: P = 0.002 3, P = 0.000 2; norepinephrine：P < 0.000 1, P < 0.000 1. (3) The p-p38/p38 ratio was significantly reduced in the fentanyl combined with propofol group and the hydromorphone combined with propofol group (P < 0.000 1, P < 0.000 1) compared with the propofol group rats. Conclusion: Hydromorphone combined with propofol can effectively inhibit the phosphorylation of p38MAPK, thereby relieving pain. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of fenofibrate on the diversity of intestinal flora in mice with nonalcoholic fatty liver disease(NAFLD). Methods A total of 30 mice were randomly divided into normal group, high-fat group, and fenofibrate treatment group, with10 mice in each group. The mice in the high-fat group and the fenofibrate treatment group were given high-fat diet intervention for 14 weeks, and those in the normal group were given normal diet for 14 weeks. After 10 weeks of dietary intervention, the mice in the fenofibrate treatment group were given fenofibrate by gavage and high-fat diet for another 4 weeks, and the change in body weight was monitored during the whole process. Fecal samples were collected after 14 weeks, and high-throughput sequencing 16 S rRNA was used to investigate the diversity and difference of intestinal flora. Liver tissue samples were collected, and HE staining and oil red O staining were performed to observe the degree of steatosis. A one-way analysis of variance was used for comparison between multiple groups, and the least significant difference t-test was used for further comparison between two groups. Results After 10 weeks of intervention, the fenofibrate treatment group had a significantly lower body weight than the high-fat group(P<0.05). The results of HE staining and oil red O staining showed that the fenofibrate treatment group had a significantly lower degree of fat deposition than the high-fat group. There was a significant difference in intestinal flora between the fenofibrate treatment group and the high-fat group, while there was no significant difference in intestinal flora between the fenofibrate treatment group and the normal group. The fenofibrate treatment group had significant increases in the abundance of Bacteroidetes, Verrucomicrobia, Faecalibaculum, Muribaculaceae_norank, and Akkermansia and significant reductions in the abundance of Firmicutes, Actinobacteria, Clostridium, Turicibacter, and Bifidobacterium. Conclusion The increases in the abundance of Bacteroidetes and Verrucomicrobia and the reductions in the abundance of Firmicutes and Actinobacteria suggest that fenofibrate may have positive significance in the treatment of NAFLD. [ABSTRACT FROM AUTHOR]

Objective To analyze the characteristic information of platelet-collecting blood donors in the main city of Chongqing,and to provide basis for evaluating the existing recruitment strategies and recruiting platelet-collecting blood donors in the future.Methods We collected the personal data of platelet donors in the main urban area of Chongqing from 2018 to 2020 through the blood management system.Then,we make statistical analysis on the gender,age,education level and occupation of this group.Results From 2018 to 2020,there were 17 229 platelet donors in the main urban area of Chongqing,with an average annual average of 5 743.The ratio of male and female blood donors was 2.44∶1.The proportion of blood donors aged 18-< 30 increased in recent years,while that of blood donors aged 50-<60 decreased yearly.The proportion of blood donors with junior high school and technical education level tended to decline,while that of blood donors with graduate education level increased.Among blood donors in different occupations,the proportion of staff, civil servants and teachers increased significantly,while that of soldiers,farmers,business services and students decreased significantly.Conclusion Among platelet donors in Chongqing main city,the proportion of elderly and low-educated blood donors tended to decrease in recent three years.In addition,there was still a problem that the proportion of female blood donors,employees of public institutions and civil servants kept low. [ABSTRACT FROM AUTHOR]

BACKGROUND: Thoracic and dorsal pain caused by osteoporotic vertebral compression fractures is very common in clinic. Conventional treatment methods include medicine, physiotherapy, calcium supplement and local nerve block. They all have certain therapeutic effects, but the pain recurs shortly after drug withdrawal. As a technique for treating this kind of pain, pulsed radio frequency has a remarkable short-term effect, but it lacks standardized treatment for osteoporosis, and its long-term effect is not good. OBJECTIVE: To investigate the therapeutic effect of pulsed radio frequency combined with guideline calcium supplement on thoracic spinal nerve root pain caused by osteoporotic vertebral compression fracture. METHODS: The 110 patients with osteoporotic vertebral compression fractures were randomly divided into two groups, with 55 cases in each group. Informed consent was obtained from patients of the two groups. This study was approved by the Hospital Ethics Committee. Pulsed radiofrequency group received pulsed radiofrequency treatment for vertebral compression fracture corresponding to dorsal root ganglion of spinal nerve. Nerve block group received nerve block treatment for corresponding dorsal root ganglion of spinal nerve. Both groups were treated with calcium carbonate D3, calcitonin and zoledronic acid for osteoporosis. Visual Analogue Scale and Oswestry Dysfunction Index results were compared between the two groups before and 1, 3, 6 and 12 months after treatment. The N-terminal propeptide of human procollagen type I and tartrate-resistant acid phosphatase isomer 5b levels were compared between the two groups. RESULTS AND CONCLUSION: (1) The Visual Analogue Scale score and Oswestry Dysfunction Index were significantly improved in both groups after 1,3, 6 and 12 months of treatment compared with those before treatment (P< 0.05). In 1 month after operation, there was no significant difference between the two groups (P > 0.05). In 3, 6 and 12 months after operation, the improvement of Visual Analogue Scale score and Oswestry Dysfunction Index in the pulsed radiofrequency group was better than that in the nerve block group (P < 0.05). (2) There was no significant difference in bone metabolism indexes N-terminal propeptide of human procollagen type I and tartrate-resistant acid phosphatase isomer 5b between the two groups at 1 month after operation and before operation (P > 0.05). N-terminal propeptide of human procollagen type I and tartrate-resistant acid phosphatase isomer 5b in the two groups were significantly improved at 3, 6 and 12 months after operation (P < 0.05). (3) Pulse radio frequency combined with guideline calcium supplement in the treatment of chest and back pain caused by osteoporotic vertebral compression fractures can not only noticeably reduce pain and improve the quality of life, but also fundamentally improve osteoporosis, with satisfactory short-term and long-term results. [ABSTRACT FROM AUTHOR]

Peroxisome proliferator-activated receptor alpha (PPARα) is a member of the nuclear receptor superfamily and plays a central regulatory role in lipid metabolism. Recent studies have found that PPARα agonists play a key role in the prevention and treatment of nonalcoholic fatty liver disease, which has also been reported in other liver diseases. This article reviews the role and mechanism of PPARα in nonalcoholic fatty liver disease, alcoholic liver disease, viral hepatitis, cholestatic liver disease, drug-induced liver injury, and hepatocellular carcinoma, so as to provide a reference for the research on the new application of conventional drugs associated with PPARα. [ABSTRACT FROM AUTHOR]

BACKGROUND: Previous studies have confirmed that both ciliary neurotrophic factor and olfactory ensheathing cells have a certain therapeutic effect on spinal cord injury, but there is no report on whether their combination treatment has a better therapeutic effect. OBJECTIVE: To explore the effect of lentiviral-mediated transplantation of olfactory ensheathing cells modified with ciliary neurotrophic factor gene in rats with spinal cord injury and its related mechanisms. METHODS: (1) In vitro experiment: There were four groups in the in vitro experiment: spinal cord neurons in control group and model group were cultured alone, while in overexpression co-culture group and normal co-culture group, Transwell chamber was used to establish the co-culture model of spinal cord neurons and olfactory ensheathing cells modified by ciliary neurotrophic factor gene or not. Alginate was given to damage the cells in the latter three groups. After 12 hours of co-culture, cell counting kit-8 assay was used to detect cell viability; qRT-PCR and western blot were used to detect the expression of BAX, BCL-2, Caspase 3 and ciliary neurotrophic factor in spinal neurons. (2) In vivo experiment: There were four groups in the in vivo experiment: sham operation group, model group, treatment group, and combination treatment group. Modified Allen’s method was used to make the rat model of spinal cord injury in the latter three groups. At 1 week after modeling, 5 μL of olfactory ensheathing cell suspension or ciliary neurotrophic factor overexpressing olfactory ensheathing cell suspension was injected 1 mm above and below the injured site in the treatment and combination treatment groups, respectively; similarly, the same volume of culture medium was given in the sham operation and model groups. At 4 weeks after treatment, Basso, Beattie and Bresnahan score and grid-climbing test were conducted to evaluate the motor ability of rats; hematoxylin-eosin staining was used to detect the pathological progression of spinal cord injury; and qRT-PCR and western blot were used to detect the expression of BAX, BCL-2, Caspase 3 and ciliary neurotrophic factor in spinal cord tissues. RESULTS AND CONCLUSION: Olfactory ensheathing cells modified with ciliary neurotrophic factor gene could significantly alleviate cell injury and spinal cord injury (P < 0.001), increase the expression of ciliary neurotrophic factor (P < 0.01) and decrease the expression of apoptosis-related molecules BAX, BCL-2 and Caspase 3 (P < 0.01). To conclude, transplantation of olfactory ensheathing cells modified by ciliary ganglion neurotrophic factor gene can improve the motor function after spinal cord injury, which may be related to the down-regulation of BAX, BCL-2 and Caspase-3 expression and reduction in the apoptosis of spinal cord neurons [ABSTRACT FROM AUTHOR]

In order to address the incomplete polymerization of different monomers resulted by their different reactivities,we perform a polymerization ofβ-lactam monomer bearing hydrophobic sidechains,3,3,4,4-tetramethylazetidine-2-one(TM)and another monomer having positively charged groups in the sidechain,3-(tert-butyloxycarbonyl aminomethyl)-4,4-dimethylazetidin-2-one(MM),which show slow reactivity during polymerization.The polymerization condition of these monomers was optimized by exploring different monomer concentrations,reaction solvents and initiators.The results indicated that initiators with potassium counter ion can initiate a faster polymerization ofβ-lactam monomers and result in a higher conversion of monomers compared with that with lithium counter ion.In addition,increasing the reactant concentration and optimizing the reaction solvent could also increase the reaction rate.The investigations on the minimum inhibitory concentration(ρMIC)of synthesized polymers revealed that the polymers obtained at the optimized reaction conditions presented slightly increased antimicrobial activities against some types of microbes [ABSTRACT FROM AUTHOR]

Patients with severe liver diseases are prone to nosocomial infection due to serious liver dysfunction, low immune function, and reduced stress ability to infection, and such infections further aggravate patients5 conditions and directly affect their clinical outcome. This article reviews the clinical features of nosocomial infection in patients with severe liver diseases and related risk factors. [ABSTRACT FROM AUTHOR]

以眼部为首发症状且单独出现的梅毒性视神经炎很少见,临床上极易漏诊或误诊。该文报道了1例双眼梅毒性 视神经炎患者的诊断、治疗,且作者通过查阅文献阐述了梅毒性眼病可出现的特征性改变。该病在诊断和治疗时,不能只局限于眼部,应兼顾全身情况,综合诊治,尽可能做到早诊断、早治疗,以免漏诊或延误治疗。 [ABSTRACT FROM AUTHOR]

以眼部为首发症状且单独出现的梅毒性视神经炎很少见,临床上极易漏诊或误诊。该文报道了1例双眼梅毒性 视神经炎患者的诊断、治疗,且作者通过查阅文献阐述了梅毒性眼病可出现的特征性改变。该病在诊断和治疗时,不能只局限于眼部,应兼顾全身情况,综合诊治,尽可能做到早诊断、早治疗,以免漏诊或延误治疗. [ABSTRACT FROM AUTHOR]

目的　探讨血清人半胱氨酸蛋白酶抑制剂S（CST4）检测对胃癌的诊断效能。方法　收集胃癌患者80例 （胃癌组），慢性萎缩性胃炎、胃息肉、胃溃疡患者50例（癌前组），浅表性胃炎患者50例（胃炎组）。采集患者空腹静 脉血，采用酶联免疫双抗夹心法定量检测 CST4，化学发光微粒子免疫法检测癌胚抗原（CEA）、糖类抗原 125 （CA125）、糖类抗原 199（CA199）；采用新型胃癌筛查（GCR）评分系统评价胃癌风险程度。比较三组上述指标的差 异，应用受试者工作特征曲线（ROC）分析各指标单独检测与联合检测对胃癌的诊断效能。结果　胃癌组 CST4、 CEA、CA125、CA199水平及GCR评分均高于癌前组和胃炎组（P均<0. 05）；癌前组GCR评分高于胃炎组（P<0. 05）， CST4、CEA、CA125、CA199水平与胃炎组比较差异无统计学意义（P均>0. 05）。ROC 曲线分析显示，单项指标检测 中血清CST4的诊断效能最高，敏感度为76. 8%，特异度为80. 1%；CST4、CEA、CA125、CA199、GCR评分联合检测诊 断胃癌的AUC高于各单项指标，敏感度为81. 9%，特异度为83. 1%。结论　血清CST4对胃癌诊断效能高于目前临 床常用的指标，其与临床常用肿瘤标志物联合应用可进一步提高胃癌的诊断率。 [ABSTRACT FROM AUTHOR]

An antimicrobial poly-β-peptide(β-amino acid polymer)was synthesized and characterized by nuclear magnetic resonance and gel permeation chromatography to give the number-average molecular weight of the polymer. The antibacterial activity of this poly-β-peptide against Gram positive bacteria, including multiple drug-resistant strains, was evaluated by its minimum inhibitory concentration(MIC). The MIC test showed that this poly-β-peptide had potent antibacterial activity(MIC value in the range of3. 13-12. 5μg/mL)against multiple strains of Gram positive bacteria, and it demonstrated the best activity toward B. subtilis with a MIC at 3. 13μg/mL. Investigation on possible antimicrobial resistance after repeated use of this antimicrobial poly-β-peptide indicated that S. aureus displayed no resistance to this poly-β-peptide. However, the MIC of norfloxacin hydrochloride(the antibiotic control)increased 124 folds in the same test, which indicated that S. aureus developed strong resistance easily against this antibiotic. The antibacterial mechanism of this poly-β-peptide against Gram-positive bacteria, especially methicillin-resistant S. aureus(MRSA), was also investigated using cell membrane depolarization assay and scanning electron microscopy. Results implied that this poly-β-peptide interacted with bacterial cell membrane and killed Gram positive bacteria by disrupting the cell membrane. [ABSTRACT FROM AUTHOR]

Objective By comparing the differences between the 2 types of fixation in the treatment of Lisfranc fracture damage, which is better with the application of the dorsal bridge plate and lateral screw and the combined application of the fixation in the treatment results. Methods A total of 62 patients with Lisfranc injury of the department from 2014 to January 31,2017 were included. The inclusion criteria included: follow up for 6 months, each case was divided into 4 groups: transverse screw group, bridging plate group, combined two group and group. The assessment included Kellgren-Lawrence osteoarthritis grading system and Wilppula classification of anatomical reduction. Results The risk of osteoarthritis at grade 3 or 4 in the combined use of bridge plate and lateral screw group was significantly increased, 3.01 times as much as the single use of the dorsal bridge plate (95% confidence interval 1.036-8.470, P=0.009). The multifactor analysis was related to the increased risk of severe osteoarthritis based on the anatomic reduction quality assessment and showed that the risk of severe anatomic reduction was 18.2 times lower than that of anatomic reduction (95% confidence interval 15.9-21.8), which was not associated with the type of fixation (P<0.005). Conclusion At the early stage, the treatment of Lisfranc fracture dislocation with plate or screw is quite effective in imaging, although plate fixation may have more complications, such as flat foot. The functional outcome should be the criteria for testing the superiority of which surgical procedure. Finally, a more complete set of classification systems for Lisfranc fracture dislocation should be developed to provide a guide for guiding the selection of internal fixation and the possible results of the clinical outcome. [ABSTRACT FROM AUTHOR]

Ｔｏ ｅｘｐｌｏｒｅ ｔｈｅ ａｄａｐｔａｂｉｌｉｔｙ ｏｆ ｎｅｗ ｓｕｇａｒｃａｎｅ, ｔｈｏｓｅ ｃｕｌｔｉｖａｒｓ ｗｉｔｈ ｂｅｔｔｅｒ ａｄａｐｔａｂｉｌｉｔｙ ａｎｄ ｐｅｒｆｏｒｍａｎｃｅ ｗｅｒｅ ｓｃｒｅｅｎｅｄ ｆｏｒ ｆｕｒｔｈｅｒ ｄｅｍｏｎｓｔｒａｔｉｏｎ ｉｎ Ｇｕｉｌｉｎ. Ｎｉｎｅ ｎｅｗ ｓｕｇａｒｃａｎｅ ｖａｒｉｅｔｉｅｓ ｏｆ ｄｅｍｏｎｓｔｒａｔｉｏｎ ｅｘｐｅｒｉｍｅｎｔａｌ ｓｔａｔｉｏｎ ｗｅｒｅｅｖａｌｕａｔｅｄ ｆｏｒ １-ｙｅａｒ ｐｌａｎｔ ｃｒｏｐ ａｎｄ ２-ｙｅａｒ ｒａｔｏｏｎ ｃｒｏｐ, ｔｅｓｔ ｍａｔｅｒｉａｌｓ ａｎｄ ｃｏｎｔｒｏｌｓ ａｄｊａｃｅｎｔ ｔｏ ｅａｃｈ ｏｔｈｅｒ ｗｉｔｈ ＲＯＣ２２ ａｓ ｃｏｎｔｒｏｌ. Ｔｈｅ ｇｅｒｍｉｎａｔｉｏｎ ｒａｔｅ, ｔｉｌｌｅｒｉｎｇ ｒａｔｅ, ｐｌａｎｔ ｈｅｉｇｈｔ, ｙｉｅｌｄ ａｎｄ ｓｕｇａｒ ｃｏｎｔｅｎｔ ｏｆ ｆｏｕｒｔｅｅｎ ｔｒａｉｔｓ ｗｅｒｅ ｏｂｓｅｒｖｅｄ ａｎｄ ｒｅｃｏｒｄｅｄ, ａｎｄ ｃｏｍｐｒｅｈｅｎｓｉｖｅｌｙ ｅｖａｌｕａｔｅｄ ｂｙ ＤＴＯＰＳＩＳ ｍｅｔｈｏｄ. Ｔｈｅ ｒｅｓｕｌｔｓ ｓｈｏｗｅｄ ｔｈａｔ Ｇｕｉｔａｎｇ ２９, Ｇｕｉｔａｎｇ ３１, Ｙｕｎｚｈｅ ０３-１９４, Ｙｕｎｔａｎｇ ６０, Ｆｕｎｏｎｇ ３８ ａｎｄ Ｙｕｅｔａｎｇ ５５ ｗｅｒｅ ｓｕｐｅｒｉｏｒ ｔｏ ＲＯＣ２２ ｉｎ ａｓｐｅｃｔ ｏｆ ｃｏｍｐｒｅｈｅｎｓｉｖｅ ａｇｒｏｎｏｍｉｃ ｔｒａｉｔｓ ａｎｄ ｙｉｅｌｄ ｔｒａｉｔｓ, Ｇｕｉｔａｎｇ ２９ ｈａｄ ｈｉｇｈｅｒ ｓｈｏｏｔｉｎｇ ｃａｎｅ, ｓｕｇａｒ ｙｉｅｌｄ ａｎｄ ｙｉｅｌｄ ｒａｔｅ, ｅｘｃｅｐｔ ｆｏｒ ｐｅｓｔｓ ｏｃｃｕｒ, ｓｕｇｇｅｓｔｉｏｎｓ ｏｎ ｓｔｒｅｎｇｔｈｅｎｉｎｇ ｐｅｓｔ ｃｏｎｔｒｏｌ ｉｎ ｔｈｅ ｆｉｅｌｄ ꎻ Ｇｕｉｔａｎｇ ３１ ｈａｄ ｍｏｒｅ ｅｆｆｅｃｔｉｖｅ ｓｔａｌｋｓ ａｎｄ ｈｉｇｈ ｓｕｃｒｏｓｅ ｃｏｎｔｅｎｔ, ｓｕｇｇｅｓｔｉｏｎｓ ｏｎ ｓｔｒｅｎｇｔｈｅｎｉｎｇ ｔｈｅ ｍａｎａｇｅｍｅｎｔ ｏｆ ｆａｔ ａｎｄ ｗａｔｅｒ ｔｏ ｉｎｃｒｅａｓｅ ｙｉｅｌｄ ｒａｔｅꎻ Ｙｕｎｚｈｅ ０３-１９４ ｈａｄ ｈｉｇｈ ｇｅｒｍｉｎａｔｉｏｎ ｒａｔｅ, ｔｉｄｙ ｓｅｅｄｌｉｎｇ, ｍｏｒｅ ｅｆｆｅｃｔｉｖｅ ｓｔａｌｋｓ, ｈｉｇｈｅｒ ｓｈｏｏｔｉｎｇ ｃａｎｅ, ｂｕｔ ｉｔｓ ｄｅａｄ ｈｅａｒｔ ｓｅｅｄｌｉｎｇｓ ａｎｄ ｓｍｕｔ ｉｎｃｉｄｅｎｃｅ ｗｅｒｅ ｒａｒｅｌｙ ｓｅｒｉｏｕｓ ｔｈａｎ ｔｈｏｓｅ ｏｆ ｏｔｈｅｒ ｃｕｌｔｉｖａｒｓ, ｓｕｇｇｅｓｔｉｏｎｓ ｏｎ ｓｔｒｅｎｇｔｈｅｎｉｎｇ ｔｈｅ ｍａｎａｇｅｍｅｎｔ ｏｆ ｄｉｓｅａｓｅ ａｎｄ ｉｎｓｅｃｔ ｐｅｓｔｓ ｉｎ ｔｈｅ ｆｉｅｌｄꎻＹｕｎｔａｎｇ ６０ ｈａｄ ｈｉｇｈｅｒ ｓｕｇａｒ ｙｉｅｌｄ ａｎｄ ｓｔｒｏｎｇ ｌｏｄｇｉｎｇ ｒｅｓｉｓｔａｎｃｅ,ｅｘｃｅｐｔ ｆｏｒ ｌｏｗｅｒ ｎｅｗ ｐｌａｎｔ ｒａｔｅ,ｓｕｇｇｅｓｔｉｏｎ ｏｎ ｉｎｃｒｅａｓｉｎｇ ｓｅｅｄｌｉｎｇ ｑｕａｎｔｉｔｙꎻＦｕｎｏｎｇ ３８ ｈａｄ ｈｉｇｈ ｓｕｃｒｏｓｅ ｃｏｎｔｅｎｔ ａｎｄ ｇｏｏｄ ｄｉｓｅａｓｅ ｒｅｓｉｓｔａｎｃｅ,ａｎｄ ｃｏｕｌｄ ｂｅ ｕｓｅｄ ａｓ ａｎ ｅｘｃｅｌｌｅｎｔ ｄｉｓｅａｓｅ ｒｅｓｉｓｔａｎｔ ｍａｔｅｒｉａｌꎻＹｕｎｔａｎｇ ５５ ｈａｄ ｈｉｇｈｅｒ ｓｈｏｏｔｉｎｇ ｃａｎｅ. Ｔｈｅｒｅｆｏｒｅ,ｔｈｅ ｓｉｘ ｓｕｇａｒｃａｎｅ ｃｕｌｔｉｖａｒｓ ａｂｏｖｅ ｓｈｏｕｌｄ ｂｅ ｆｕｒｔｈｅｒ ｅｘｔｅｎｔｅｄ ａｎｄ ｄｅｍｏｎｓｔｒａｔｅｄ ｉｎ Ｇｕｉｌｉｎ. [ABSTRACT FROM AUTHOR]

To solve the soil heap problem before digging shovel of Panax notoginseng mechanical harvesting,a new type of combined digging shovel with triangular plane shovel and soil crushing shovel was designed and investigated under the premise of not reducing harvest rate.The regression analysis and the response surface analysis were conducted by Design-Expert 8.0.6 Software.The structure and the optimum working parameters of the combined digging shovel were determined with shovel surface angle of 20°, buried gap angle of 12° and operation speed of 0.75 m·s-1. The results of field experiment show that the yield rate is 98.38％, and the correctness of regression model is verified to meet the operation requirements. The harvest effect comparison of the combined digging shovel with the common digging shovel shows that the combined digging shovel is more suitable for Panax notoginseng harvesting with better performance of harvest rate and problem solving of soil heap before digging shovel. [ABSTRACT FROM AUTHOR]

To reveal the effects of nicotinamide phosphoribosyltransferase (Nampt) and high glucose and high insulin (HG+HI) microenvironment on proliferation and metastasis of human cisplatin-resistant lung cancer cell lines. Human cisplatin resistant cell line A549/DDP was divided into 6 groups (n=6): control group, high glucose and high insulin intervention group (HH, cultured with 30 mmol/L glucose and 500 mU/L insulin for 72 h), sh-NC and sh-Nampt transfected groups (Lipofectamine 2000 was used to transfect sh-NC and sh-Nampt into cells respectively, the transfection time was 48 h), HH intervention sh-NC and sh-Nampt groups (HH+sh-NC and HH+sh-Nampt). Each group had 6 replicates. The transfection efficiency was detected by qRT-PCR. Cell proliferation was detected by MTT method, apoptosis was detected by flow cytometry, cell migration and invasion were detected by Transwell, Nampt mRNA was detected by qRT-PCR, and protein expression of Nampt, Bcl-2, Bax, MMP-2, MMP-9, p-PI3K, PI3K, p-AKT and AKT were detected by Western blot. Compared with control group and sh-NC group, the expression level of Nampt mRNA and protein, relative cell viability, the number of migratory and invasive cells decreased, the rate of apoptosis increased, the expression level of Bcl-2, MMP-2, MMP-9, Nampt, p-PI3K and p-AKT protein decreased, and the expression level of Bax protein increased in sh-Nampt group (P<0.05). Compared with control group and sh-NC group, the expression level of Nampt mRNA and protein, relative cell viability, the number of migratory and invasive cells increased, the expression level of Bcl-2, MMP-2, MMP-9, Nampt, p-PI3K and p-AKT protein increased, and the expression level of Bax protein decreased in HH group and HH+sh-NC group (P<0.05). Compared with HH group and HH+sh-NC group, the expression level of Nampt mRNA and protein, relative cell viability, the number of migratory and invasive cells decreased, the rate of apoptosis increased, the expression level of Bcl-2, MMP-2, MMP-9, Nampt, p-PI3K and p-AKT protein decreased, and the expression level of Bax protein increased in HH+sh-Nampt group(P<0.05). High glucose and high insulin microenvironment may induce proliferation and metastasis of human cisplatin-resistant lung cancer cells by up-regulating Nampt/PI3K/AKT signaling pathway. [ABSTRACT FROM AUTHOR]

Litchi (Litchi chinensis Sonn.), a tropical/subtropical fruit originally from China, is cultivated all over the world with warm climates. It has become one of the most popular fruits in the world due to its delicious flavor and abundant nutrition. As a good source of minerals, dietary fiber, and phytochemicals, litchi has been employed in traditional Chinese medicine. In addition, litchi has also been used to produce various types of health products and foods, such as medicinal beverages, drinks, or soups. Litchi fruit’s pericarp tissues contain a lot of flavonoids, polysaccharides, and various proteins. Polysaccharides are a kind of biomacromolecules composed of more than 10 monosaccharide units, which generally exist in plants, animals, algae and microorganisms such as fungi and bacteria. Some investigations have indicated that Litchi chinensis polysaccharides have strong antioxidative activity and can be explored as novel potential antioxidants. Plant polysaccharides are excellent candidates for health and therapeutics, and searching for polysaccharides with antioxidant activity from plant resources has become a hot research topic. Furthermore, the polysaccharides exhibit good hygroscopicity and moisture retention activity due to the presence of a large number of hydroxyl or carboxyl polar groups, which can form hydrogen bonds with water molecules and cross-linked reticular structure. Antioxidant activity and hygroscopicity and moisture retention activity are important for healthcare to protect mankind from the attack of free radicals and retard the initiation of many chronic diseases. Therefore, it is necessary to more effectively purify and determine the physicochemical properties of polysaccharides from Litchi chinensis. In the current research, crude polysaccharides were extracted from Litchi chinensis to obtain the polysaccharide fraction (PLC). PLC was further purified and a kind of homogenous hetero polysaccharide (PLC-1) was isolated. The physicochemical properties, antioxidant activity, hygroscopicity and moisture retention activity of PLC-1 were analyzed. The polysaccharides of Litchi chinensis were extracted and purified by ultrasonic-assisted extraction, alcohol precipitation, deprotein, decolouring, dialysis, DEAE-cellulose 52 and Sephadex G-100 gel column chromatography. Purity was determined by ultraviolet-visible spectral scanning, spin spectrophotometry and gel filtration chromatography (GPC). The relative molecular mass was determined by high-performance gel permeation chromatography. The monosaccharide composition was analyzed by HPAEC-PAD. The antioxidant activity was evaluated on the basis of DPPH, OH free radical scavenging activity. The hygroscopicity and moisture retention activity was evaluated by adopting the in vitro method. PLC-1 was isolated and purified from Litchi chinensis, which was a kind of homogeneous polysaccharide. The relative molecular mass of PLC-1 was 2.35 × 104 Da. The monosaccharide of PLC-1 included galactose, rhamnose and glucose with a molar ratio of 1.00:3.52:5.89. The antioxidant activity showed PLC-1 had a good dose-effect relationship on DPPH radical and hydroxyl radical in a certain concentration range, and the 50% inhibiting concentration (IC50) of DPPH and hydroxyl radical scavenging activity was 0.41 and 0.31 mg/mL, respectively. The hygroscopicity and moisture retention activity indicated PLC-1 had good effect. Due to the physicochemical properties, antioxidant activity, hygroscopicity and moisture retention activity, PLC-1 has a good prospect in the preparation of functional food and cosmetics. [ABSTRACT FROM AUTHOR]

Plastic films have been used extensively as mulching for crops in modern agriculture, and remarkably improved crop yields in the past decades. However, the vast amount of residual plastic films results in serious pollution in the arable land. In order to expound the status of residual films and the residual coefficient of mulch in typical croplands in North China, the amounts of the residual films were studied by using the methods of farm survey with questionnaires and quadrat sampling in 124 study sites from 2008 to 2011 in Beijing, Tianjin, Hebei, Shandong, Shanxi, Henan, Inner Mongolia. During questionnaire survey, the relevant status of mulching application including soil characteristics, crop type, mulching time and recovery method etc. was investigated. Based upon this, the residual coefficient of typical crops (peanut and cotton) was calculated through the methods of field monitoring from 2011 to 2014, by determining the amount of residual film before and after test. The results showed that the amount of film mulch residue in North China fields ranged from 0.2 to 82.2 kg/hm2, with an average value of 26.8 kg/hm2. Among the surveyed sites, 4.8% of fields presented a greater mulch residual volume than the national film residue standard (65 kg/hm2), and 16.1% of fields showed a residual film amount approaching this threshold. The residual amount was significantly different (P＜0.05) between different crops and provinces. The residual amount of mulch film in peanut and cotton field was highest, reaching 32.0 and 31.8 kg/hm2, respectively. The high residue film in peanut and cotton field was caused by different reasons. For peanut, it was attributed to the small mulching thickness which was mostly less than 0.005 mm, while for cotton the reason was the long mulching time. Meanwhile, Hebei was the one with the most residual mulching film (36.8 kg/hm2), because of the large proportion of peanut and cotton fields. In the year of 2011, the total amount of residual film in North China was 1.5×105 t. Vegetables, cotton and peanut field had great contributions to the total amount of residual film in this region, and the amount of residual film was 5.5×104, 3.9×104 and 3.0×104 t, respectively. Three years of residual coefficient test showed that the residual coefficient of peanut and cotton were 9.7% and 14.3%. If the present mulching pattern was carried on, the quantity of residual film in peanut and cotton fields would reach up to 69.1 and 70.4 kg/hm2 in 2021, becoming polluted areas that exceed the national standard of film residue. Although the pollution of residual film was not serious at present, it will become a significant issue if no measure was taken to solve it. It is in urgent need to elevate mulch film standards, develop biodegradable mulch film and enhance mulch film recycling. This study will eventually contribute to promoting effective control and management of residual mulch pollution in North China. [ABSTRACT FROM AUTHOR]

In the traceability system of Chinese herbal medicine quality, due to the characteristics of Chinese herbal medicines, we need the shared ownership of radio frequency identification (RFID) tags between the Chinese herbal parties. Because Chinese herbal medicines have unique features as compared to other ordinary commodities, we should put forward higher requirements for the security of RFID tag’s data based on the consideration of medicines quality and safety. In the process of the ownership transfer of RFID tags, the transfer protocol must have the advantages of high security, being able to withstand external attacks and prevent leakage of privacy data for real traceability of Chinese herbal medicines. But common ownership transfer protocols are mostly related to the ownership transfer process with a single tag, and have prevalence of privacy data leakage, ownership transfer process instability and other shortcomings. How to achieve the transfer protocol of the shared ownership becomes the emphasis to ensure data security focused on RFID tag, and also becomes the important key to the quality guarantee of Chinese herbal medicines. For the defects of existing ownership transfer protocol with single label, using TTP (trusted third party) symmetric encryption mechanism, we put a new shared ownership transfer protocol i.e. TTP model shared ownership transfer protocol (TSOTP) based on the lightweight encryption algorithm. After the process of initial label certification by the TTP certification authority, we use the symmetric encryption algorithms to produce the symmetric key of generation group. The new owner of the tags uses a shared group key to achieve authentication, and then assigns a new key for the RFID tag which is ultimately authorized; when the new owner of the tags receives the updated label key to confirm the results using hash function operation with the information of the backend server received,then detecting the legality of the shared key, he can read the sensitive data of label. In the implementation of RFID authentication protocol, the calculation of certification and response process will consume a lot of system resources, and how to reduce the amount of calculation becomes a key for the efficiency of protocol operation. After the reader receives the confirmation label, the label will generate a random number by the XOR,the result is encrypted by hash function and to compared with the received information for the previous step,if they get on matching,it will be sent a responsed information to the server,otherwise it will terminate implementation of the agreement in order to avoid the occurrence of the phenomenon of replay attacks. TSOTP protocol can improve the stability of the tab in the transfer process of ownership, well achieve the safe transfer of the shared ownership among users, ensure data security of RFID tags, improve forward and backward security, reduce private data leaks, Dos attacks, replay attacks and other risks, and meanwhile avoid the repeated transfer of ownership, simplify the calculation amount of label certification, which shows good stability and efficiency and meets the research needs of Chinese herbal medicine quality traceability system. Malicious attackers frequently tamper tag data and collect herbs in the transfer process in order to achieve the purpose of obtaining illegal profits. How to improve security of the RFID tags for Chinese herbal’s circulation will become the research focus in the future process of medicine traceability system establishment. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the clinical efficacy of heparin combined with cefuroxime sodium in treatment of central venous catheter infection in hemodialysis patients. METHODS: 46 hemodialysis patients with central venous catheter infection admitted into Hongqi Hospital Affiliated to Mudanjiang Medical College from Jan. 2015 to Jan. 2017 were extracted to be divided into observation group and control group via the random number table, with 23 cases in each. The control group was treated with heparin combined with gentamicin, while the observation group received heparin combined with cefuroxime sodium. The clinical efficacy, complications, placement time of tube and extubalion ratio of two groups were observed. RESULTS: The total effective rale of observation group was 95. 65% (22/23), significantly higher than that of control group (73.91%, 17/23), with statistically significant difference (P <0. 05). The incidence of complications of observation group was 4. 35% (1/23), significantly lower than that of control group (26.09%, 6/23), with statistically significant difference (P < 0.05). The average placement time of tube in observation group was (62. 28 ± 11.09) min, significantly longer than that in control group [(49. 98 ± 10. 87) min] ; the extubalion ratio in observation group was 4. 35% (1/23), significantly lower than that in control group (26.09%, 6/23), with statistically significant difference (P < 0.05). CONCLUSIONS: Heparin combined with cefuroxime sodium in treatment of central venous catheter infection in hemodialysis patients can reduce the incidence of infection, prolong the placement time of tube and enhance the efficacy of hemodialysis. [ABSTRACT FROM AUTHOR]

Objective: To improve the rate of rational use of intravenous drugs and reduce the number of unreasonable medical orders in the pharmacy intravenous admixture service (PIVAS). Methods: From July, 2016, the numbers of unreasonable medical orders and rejected medical orders from various wards of the hospital were calculated by the prescription review pharmacists and the feed-back system was thus established. Rejected medical orders prescribed during the period from July 2015 to June 2017 were collected from the PIVAS of Changhai Hospital of the Second Military Medical University, and changes in irrational medical orders 12 months before and after the establishment of the pharmacist intervention feed-back system were compared. Results: From July 2015 to June 2016, pharmacists in the PIVAS handled a total of 4 423 306 infusion bags, and 17 079 bags were rejected, accounting for 0.39%, while from July 2016 to June 2017,4 648 757 bags were handled, and 11 558 bags were rejected, accounting for 0.25%. Statistical significance could be seen, when comparisons were made between them (PC0.01). Conclusion: The feed-back system produced good effect and the number of unreasonable medical orders was considerably reduced, furthermore, the level of pharmaceutical care was obviously enhanced through effective communication between physicians and pharmacists. [ABSTRACT FROM AUTHOR]

目的 观察盐酸多奈哌齐联合尼莫地平治疗血管性痴呆患者的临床疗效。方法 将152例血管性痴 呆患者随机分为两组各76例,均按照脑血管病二级预防进行常规治疗;对照组给予尼莫地平治疗，观察组在对照 组基础上另予盐酸多奈哌齐治疗，连续服用8周。治疗前及治疗后第4、8周，分别采用蒙特利尔认知评估量表 (MoCA)及简易精神状态检查(MMSE)评价患者的认知功能，采用临床痴呆量表(CDR)评价患者的痴呆程度，采用 日常生活活动能力量表(ADL)评价患者的日常生活自理能力；治疗第8周评价临床疗效，记录不良反应。结果 两组治疗前河MMSE,MoCA,CDR,ADL评分比较，差异无统计学意义。两组治疗后第4、8周MMSE、MoCA评分均较 治疗前升高(P均<0. 05)，而CDR、ADL评分均较治疗前降低(P均<0.05);观察组治疗后MMSE、MoCA评分均较 高于对照(P均<0. 05)，而CDR、ADL评分均低于对照组（P均<0.05)。观察组显效率40. 8%、总有效率93.4%， 对照组分别为18.4%、85.5%，观察组显效率高于对照组(P<0.05)。两组不良反应发生率比较，差异无统计学意 义：结论盐酸多奈哌齐联合尼莫地平治疗血管性痴呆患者能够提高临床治疗效果，改善患者的认知能力、痴呆 程度以及日常生活能力。 [ABSTRACT FROM AUTHOR]

Fever is defined as an elevation of body temperature above the normal range and it's one of the most common medical signs in the neurosurgical intensive care unit (NICU). Fever is a protective response of the human body toward harms, yet it also increases the burden of human body which may lead to a detrimental clinical outcome. The reasons lead to fever in NICU are complicated and interplayed and there lacks specific clinical manifestation of each kinds of fever. So it's critical to understand the causes, treatment and prognosis of fever systematically. Though there lies great studies of fever, it lacks systematic description of fever in NICU A review of the literature was compiled through MEDLINE and our own clinical experience are presented in order to have a thorough understanding of the fever in NICU. [ABSTRACT FROM AUTHOR]

Objective To analyze the predictive factors for rapid virologic response (RVR) in patients with chronic hepatitis C (CHC) who received combination therapy with pegylated interferon (PEG-IFN) and ribavirin. Methods A total of 127 CHC patients who were admitted to our department from 2010 to 2012 and received PEG-IFN combined with ribavirin were enrolled in this retrospective cohort study. The patients were divided into RVR group and non-RVR (NRVR) group according to their virologic responses after 4 weeks of antiviral therapy. Demographic characteristics and the clinical features prior to treatment were compared between the two groups, and the potential factors that contributed to the acquisition of RVR were analyzed. Comparison of categorical data between groups was made by chi-square test, predictive factors were analyzed by nonparametric test for two independent samples (Mann-Whitney U test), independent predictive factors were tested by univariate and multivariate logistic regression analyses, and the continuous variables of predictive factors were analyzed using receiver operating characteristic curves. ResultsOf the 127 CHC patients, 86 were males and 41 females. There were 11 confirmed cases of liver cirrhosis. There were 100 patients (78.74%) who achieved an RVR, and 27 (21.26%) with NRVR. Nonparametric analysis showed that eight factors, which were age, time of infection, level of pre-treatment alanine aminotransferase, level of pre-treatment hyaluronic acid, development of hypertension, type of interferon, pathway of infection, and hepatitis C virus (HCV) genotype, were significantly different between the RVR and NRVR groups (P＜0.05). The logistic regression analysis identified the following factors as independent predictive factors for RVR: non-genotype 1 (OR: 0.203, 95%CI: 0.051-0.802, P＜0.05), time of infection (OR: 0.925, 95%CI: 0.868-0.987, P＜0.05), and absence of hypertension (OR: 0.129, 95%CI: 0.032-0.521, P＜0.05). Conclusion Patients with shorter history of HCV infection, absence of complicated hypertension, and non-genotype 1 HCV infection have an increased likelihood of achieving an RVR. [ABSTRACT FROM AUTHOR]

To explore the prognostic value of cuproptosis-related genes in cervical cancer, the clinical data of cervical cancer patients were downloaded from the TCGA database and randomly divided into training group and validation group. Cuproptosis-related genes were screened out by univariate Cox, LASSO-Cox and multivariate Cox analysis, and the risk model was constructed. Overall survival (OS) of the two subgroups and the entire cohort was analyzed by Kaplan-Meier curve, and the prognostic value of the model was verified by ROC curve and PCA. Univariate and multivariate analyses were performed to evaluate the independent prognostic value of clinical features and risk scores. The biological functions and pathways between the two subgroups were analyzed by gene ontology (GO) and Kyoto encyclopedia enrichment of genes and genomes (KGEE), and the sensitivities of the two subgroups to drugs were further analyzed. Finally, prognostic models of five cuproptosis-related genes (FDX1, ARF1, APP, HSF1, MT1A) were constructed. From the survival curve of risk score, OS in the low-risk group was much higher than that in the high-risk group, and the prognosis was good (P<0.05). By univariate and multivariate Cox analysis, risk score an independent prognostic factor (P<0.001). The predictive power of the prognostic model was demonstrated by receiver operating characteristic curve (ROC) and principal component analysis (PCA). ROC curve analysis was used to assess the sensitivity and specificity of risk scores and other clinical features, such as age, grade, and stage. And the results showed that the prognostic value of risk scores was superior to other clinical features. The results of enrichment analysis showed that gene function was mainly concentrated in extracellular matrix and extracellular structure. According to TIDE algorithm, immunotherapy efficacy of patients in the low-risk group was superior to that in the high-risk group. In addition, significant differences were found in the sensitivity of 24 drugs between the two subgroups. The study established a prognostic risk model composed of 5 cuproptosis-related genes, and proved that the model can accurately predict the prognosis of patients. And patients with low risk score are more likely to benefit from immunotherapy, which provides theoretical basis for clinical individualized therapy. [ABSTRACT FROM AUTHOR]

Objective : To investigate antibacterials stock in the sample hospitals,so as to provide solid evidence for the monitoring and control of antibacterials in clinical application by medical institutions and administrative departments. Methods : With the support from the monitoring and control center of antibacterials in clinical application ,the status of antibacterials stock in 156 hospitals was statistically analyzed. Results : The antibacterials stock of these hospitals included 11 categories containing 150 varieties and 470 items,totaling 2242 products. Conclusion : The antibacterials stock in the sample hospitals was wide and varied. Attention should be paid to safety risks and the structure of various antibacterials should be optimized. [ABSTRACT FROM AUTHOR]

目的观察骨髓基质细胞衍生因子1(SDF-1)在慢性髓性白血病(CML)不同病程中的表达,并探讨其与临床参数的关系。方法选择CML患者30例及健康对照者12例,CML患者30例中,初治慢性期10例、慢性期获得主要分子学反应(MMR)10例(患者接受一代或二代TKI治疗)、急变期10例(接受TKI治疗疾病仍发生进展)。抽取骨髓,提取骨髓单个核细胞,采用实时定量PCR技术检测SDF-1 mRNA,比较CML与健康对照之间及不同病程CML患者之间SDF-1的表达差异,分析SDF-1 mRNA表达水平与CML患者临床参数间的关系。选取上述患者中CML初治慢性期患者6例、急变期4例的骨髓活检标本作为慢性期组及急变期组,并应用5例份无明显病变的骨髓活检标本作为对照组,应用免疫组化技术检测骨髓组织SDF-1蛋白的表达。结果与健康对照比较,CML初治慢性期患者、急变期患者骨髓单个核细胞SDF-1 mRNA表达水平降低(P均<0.05),CML慢性期治疗后获得MMR者SDF-1 mRNA水平与健康对照比较,P>0.05;CML急变期患者SDF-1 mRNA表达水平较CML初治慢性期患者高(P<0.05)。急变期组骨髓组织中SDF-1表达阳性率高于慢性期组(P<0.05),慢性期组与对照组比较P>0.05。SDF-1 mRNA低表达(<4.36×10-3)患者外周血白细胞计数高于SDF-1 mRNA高表达(≥4.36×10-3)者(P<0.05),而SDF-1 mRNA的表达与患者年龄、性别、血红蛋白、血小板计数、CD34+细胞比例、BCRABL1/ABL1、染色体核型均无相关性(P均﹥0.05)。结论 CML患者骨髓微环境中SDF-1表达降低,其水平降低与病情加重及白细胞升高有关。 [ABSTRACT FROM AUTHOR]

目的 观察自拟宜胃止痛抗幽汤辅助治疗幽门螺杆菌（HP)感染性胃脘痛的效果。方法将165例HP 感染性胃脘痛患者随机分为对照组82例和观察组83例。两组均给予奥美拉唑肠溶片、莫沙必利分散片、硫糖铝 混悬液、阿莫西林克拉维酸钾片治疗，观察组另口服自拟宜胃止痛抗幽汤加减治疗,3周为1个疗程。观察两组症 状积分、主要症状缓解情况和Hp清除率、中医脾胃系统疾病PRO量表和治疗效果。结果观察组治疗后胃脘痛、 痞满、嗳气、纳差缓解率和Hp清除率差异均有统计学意义(P均<0.05)。两组治疗后生理领域、独立性领域、心理 领域、社会和自然领域评分及总分均低于治疗前(P均< 0. 05);观察组治疗后上述各单项评分与对照组比较差异 无统计学意义(P均> 0.05),但总分低于对照组（P < 0. 05)。观察组临床治疗总有效率高于对照组，差异有统计 学意义(P <0.05)。结论自拟宜胃止痛抗幽汤辅助治疗Hp感染性胃脘痛可有效缓解患者临床症状，提高Hp 清除率及临床疗效。 [ABSTRACT FROM AUTHOR]

目的观察奥沙利铂、氟尿嘧啶筛选前后胃癌SGC7901细胞株中CD44v6/ATP结合转运蛋白G超家族成员2(ABCG2)表达变化。方法取对数生长期的胃癌SGC7901细胞株,分别用0、4、12、20、28、36μg/m L的奥沙利铂及0、30、50、70、90、110μg/m L的氟尿嘧啶进行干预,MTT法得到奥沙利铂、氟尿嘧啶作用于实验细胞株的最适浓度分别为30、28μg/m L。以最适浓度作用于实验细胞株分离出胃癌干细胞,以免疫细胞化学法、半定量RTPCR法、Western blotting法检测以上两种药物筛选前后细胞株中CD44v6/ABCG2的阳性表达率。再以单细胞悬浮成瘤实验、免疫细胞化学法检测次代干细胞级别的实验细胞株中CD44v6/ABCG2的阳性表达率。结果奥沙利铂、氟尿嘧啶筛选的干细胞级别的实验细胞株中CD44v6/ABCG2阳性表达率明显上调,能在低浓度血清培养基中形成悬浮肿瘤球,免疫细胞化学检测其次代细胞中CD44v6/ABCG2的阳性表达率又回到筛选前水平。结论奥沙利铂、氟脲嘧啶筛选后的细胞株中CD44v6/ABCG2阳性表达率明显上调,细胞株具有干细胞的特性。 [ABSTRACT FROM AUTHOR]