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Objective: To determine the effect and mechanism of ultrasound with different intensity on uterine contraction of pregnant rats and nerve injury of fetal rats. Methods: 32 pregnant rats were randomly divided into A(0) group, B(2) group, C(4) group and D (8) group, with 8 rats in each group. Rats in these groups were respectively received ultrasonic irradiation with different intensities of 0 W cm-2, 2 W cm-2, 4 W cm-2 and 8 W cm-2 for 20 min. The uterine contraction and ATPase activities of uterine smooth muscle were recorded in pregnant rats, and neuronal apoptosis and activities of cholinergic nervous system-related enzymes in cerebral cortex and hippocampus were detected in fetal rats. Results: Ultrasound increased frequency, amplitude, tension and activity of uterine contraction (P<0.05), and decreased the activities of Na+-K+ ATPase, Ca2+ ATPase and Ca2+-Mg2+ ATPase in uterine smooth muscle of pregnant rats (P<0.05). Ultrasound reduced Bcl-2 levels (P<0.05) and increased the levels of Bax and Caspase-3 (P<0.05) in the cerebral cortex and hippocampus of fetal rats. Results: Ultrasound promoted acetylcholinesterase activity, and reduced acetylcholine and acetylcholine transferase levels (P<0.05). Ultrasounds with 4 W cm-2 and 8 W cm-2 were more effective than with 2 W cm-2 ultrasound on the effect of these indicators. Conclusions: W cm-2 and 8 W cm-2 might promote the contraction of uterine smooth muscle in pregnant rats through reducing the activity of ATPases, and resulting in damage to cerebral cortex and hippocampal neurons of fetal rats, the mechanism may be associated with the imbalance of the cholinergic neuron system. 2 W cm-2 Ultrasound has little damage to fetal rat neurons. [ABSTRACT FROM AUTHOR]

Objective • To explore the uterine microbiota in women undergoing abortion and non-pregnant women. Methods • Fifty-six women who had experienced spontaneous abortion and 39 women who had experienced artificial abortion were selected to obtain the decidual tissuescurettage vacuum aspiration, and 10 non-pregnant women (without infection) were selected to obtain the endometrial tissues through gynecological surgery Department of Gynecology in Huadong Hospital. Three groups of samples were subjected to high-throughput sequencing based on the V4 variable region of the 16S ribosomal RNA (rRNA) gene, and the bacterial composition of each group of samples was analyzed. The diversity of uterine microbiota was compared among three groups. Results • Low-abundance microbiomes were detected the decidual tissues of abortion women and the endometrial tissues of non-pregnant women. Proteobacteria was the main phylum of the three groups, and Escherichia/Shigella was the main genus. The diversity of uterine microbiota in spontaneous abortion group was higher than that in artificial abortion group. Conclusion • There is a low-abundance uterine microbiota mainly composed of Proteobacteria. The dominant bacteria at the level of phylum, family and genus has no significant difference between the spontaneous abortion group and the artificial abortion group, but there is higher flora diversity in the spontaneous abortion group than in the artificial abortion group. [ABSTRACT FROM AUTHOR]

In recent decades, polycrystalline diamond composite (PDC) bit has been rapidly developed, and becomes the main drill bit in oil drilling. However, the PDC hit is easy to he adhered by the debris in the mud layer, which leads to the failure of the drill and the accident of the well. In this paper, for PDC bit and mud lithic adhesion problems, biological non smooth surface technology is used to drill mud package control, through of natural organisms surface nonsmooth surface morphology analysis. A non-smooth surface model is established; non-smooth surface anti adhesion performance is tested, indoor experiment is carried out under the different forms of non-smooth surface specimen antiadhesion properties. This research provided new ideas and methods for the prevention and treatment of PDC bit balling. [ABSTRACT FROM AUTHOR]

In the process of reverse circulation drilling, compressed air goes through a reverse circulation experiment device to monitor the flow and pressure parameters. Then it is along the annular gap of the double wall drill pipe into the bottom of the well, through rotary injection hole of the reverse circulation bit, to form the rotating upwelling that has pumping action. Finally, cuttings are carried back to the surface along the center channel. According to the principle of reverse circulation drilling and structure of drilling tools, reverse circulation experiment device was developed. The experimental device can simulate the movement process of drilling fluid in the downhole, and verify and evaluate the effect of reverse circulation. The development of the reverse circulation experiment teaching device improved the experimental teaching of the air reverse circulation drilling technology and could stimulate the interest of the students to study the structure of the reverse circulation drilling tools. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between serum vascular cell adhesion molecule-1 (VCAM-1), plasminogen activator inhibitor-1 (PAI-1), angiopoietin like protein 8 (ANGPTL8) and pregnancy outcome in patients with gestational diabetes mellitus( GDM). Methods: 136 pregnant women with GDM who were treated in The Second Affiliated Hospital of Shaanxi University of traditional Chinese Medicine from April 2019 to March 2021 were selected as the study group. 120 healthy pregnant women who came to our hospital for prenatal examination at the same time were selected as the control group. The demographic and clinical data of patients in the study group were collected, the levels of serum VCAM-1, PAI-1 and ANGPTL8 in two groups of pregnant women were detected by enzyme- linked immunosorbent assay. The pregnancy outcome of pregnant women in the two groups were observed. According to the pregnancy outcome, the pregnant women in the study group were divided into poor pregnancy outcome group and good pregnancy outcome group. Univariate and multivariate Logistic regression were used to analyze the influencing factors of adverse pregnancy outcome in GDM pregnant women. Results: The levels of serum VCAM-1, PAI-1 and ANGPTL8 in the study group were higher than those in the control group (P<0.05). The total incidence of adverse pregnancy outcome in the study group was higher than that in the control group (P<0.05). Univariate analysis showed that the poor pregnancy outcome of pregnant women with GDM were related to age, prenatal body mass index (BMI), glycosylated hemoglobin (HbAlc), fasting blood glucose (FBG), fasting insulin (FINS), total cholesterol (TC), insulin resistance index (HOMA-IR), family history of diabetes, history of childbirth, place of residence, VCAM-1, PAI-1 and ANGPTL8 levels (P<0.05). Multivariate Logistic regression analysis showed that age ≥ 35 years old, prenatal BMI ≥ 28 kg/m2, high HbAlc level, city of residence, elevated levels of VCAM-1, PAI-1 and ANGPTL8 were all influential factors of poor pregnancy outcome in pregnant women with GDM (P<0.05). Conclusion: Serum levels of VCAM-1, PAI-1 and ANGPTL8 in pregnant women with GDM are abnormally elevated, and all three are influential factors of pregnancy outcome in pregnant women with GDM, which deserved clinical attention. [ABSTRACT FROM AUTHOR]

It has been clarified that GeBP transcription factor regulates the growth and development of plant epidermal hair and participates in the control of plant leaf development. The bioinformatics methods were used to identify the GeBP gene family in the whole Glycin max(soybean)genome, and from physicochemical properties of amino acids, as well as gene structure, physical distribution of chromosomes, phylogenetic tree, and multiple sequence comparison, the functional domain, tissues expression and other basic characteristics of GmGeBP gene family were analyzed. The results were as follows:(1)A total of nine members of GmGeBP transcription factor gene family were identified, of which only two genes contained introns and all had only one intron, indicating that the gene structure of the family members is relatively simple but stable.(2)The molecular weights of GmGeBPs were 39.65-49.24 kD, and the theoretical isoelectric point was 4.65-9.08; these members were basically acidic amino acids, which were hydrophilic and unstable proteins.(3)The chromosome physical distribution showed that nine genes were unevenly distributed on seven chromosomes, two GeBP genes on chromosome 10 and 20 respectively, and one gene on chromosome 3, 5, 13, 15 and 19, respectively.(4)The phylogenetic analysis showed that GeBP members of Glycin max and Arabidopsis thaliana were closely related, clustered into four branches respectively, but far away from Oryza sativa.(5)The analysis of domains showed that all the nine GmGeBP members contained DUF573 domain, which was probably the domain interacting with cis-acting elements of target genes in GeBP transcription factors.(6)By analyzing the expression of GmGeBP transcription factor gene family, we found that the expressions of different genes in different tissues were different, with a certain specificity. The analysis and identification of GmGeBP transcription factor gene family provide the theoretical basis for further studying the molecular role of Glycin max epidermal development. [ABSTRACT FROM AUTHOR]